LOCUS       sequence-127546-       10294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP8
FEATURES             Location/Qualifiers
     source          1..10294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1420..1799
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1800..2003
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1954..1974
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2005..2023
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2026..2044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2054..2078
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2151..2160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2160..2183
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2196..2222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2238..2262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5617..5641)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5660..6159
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5695..5718)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6092..6111
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6180..6779
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6180..6199)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6676..6696
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    7365..7381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7389..7395,7396..7413,7414..7419))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7434..7455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7572..7589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7743..8331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7823..7842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8502..9293,9294..9362))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9125..9144
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9363..9467)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9535..9553
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9591..9613)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9713..9732
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9926..9948
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10076..10095
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatccttg acattgatta ttgactagtt
     1441 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     1501 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1561 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1621 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     1681 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1741 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     1801 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     1861 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1921 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     1981 gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata
     2041 gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     2101 gttttgacct ccatagaaga caccgggacc gatccagcct ccggggatct gccaccatgg
     2161 actacaagga tgacgatgac aagctcgatg gaggataccc atacgatgtt ccagattacg
     2221 ctgctcgagg ttatcaaaca agtttgtaca aaaaagttgg catgcctgct gtggcttcag
     2281 ttcctaaaga actctacctc agttcttcac taaaagacct taataagaag acagaagtta
     2341 aaccagagaa aataagcact aagagttatg tgcacagtgc cctgaagatc tttaagacag
     2401 cagaagaatg cagattagat cgtgatgagg aaagggccta tgtactatat atgaaatacg
     2461 tgactgttta taatcttatc aaaaaaagac ctgatttcaa gcaacagcag gattatttcc
     2521 attcaatact tggacctgga aacatcaaaa aagctgtcga agaagctgaa agactctctg
     2581 aaagccttaa attaagatat gaagaagctg aagtccggaa aaaacttgag gaaaaagaca
     2641 ggcaggagga agcacagcgg ctacaacaaa aaaggcagga aacaggaaga gaggatggtg
     2701 gcacattggc taaaggctct ttggagaatg ttttggattc caaagacaaa acccaaaaga
     2761 gcaatggtga aaagaatgaa aaatgtgaga ccaaagagaa aggagcaatc acagcaaagg
     2821 aactatacac aatgatgacg gataaaaaca tcagcttgat tataatggat gctcgaagaa
     2881 tgcaggatta tcaggattcc tgtattttac attctctcag tgttcctgaa gaagccatca
     2941 gtccaggagt cactgctagt tggattgaag cacacctgcc agatgattct aaagacacat
     3001 ggaagaagag ggggaatgtg gagtatgtgg tacttcttga ctggtttagt tctgccaaag
     3061 atttacagat tggaacaact ctccggagtc tgaaagatgc acttttcaag tgggaaagta
     3121 aaactgtcct gcgcaatgag cctttggttt tagagggagg ctatgaaaac tggctccttt
     3181 gttatcccca gtatacaaca aatgctaagg tcactccacc cccacgacgc cagaatgaag
     3241 aggtgtctat ctcattggat tttacttatc cctcattgga agaatcaatt ccttctaaac
     3301 ctgctgccca gacgccacct gcatctatag aagtagatga aaatatagaa ttgataagtg
     3361 gtcaaaatga gagaatggga ccactgaata tatcaactcc agttgaacca gttgctgctt
     3421 ctaaatctga tgtttcaccc ataattcagc cagtgcctag tataaagaat gttccacaga
     3481 ttgatcgtac taaaaaacca gcagtcaaat tgcctgaaga gcatagaata aaatctgaaa
     3541 gtacaaacca tgagcaacaa tctcctcaga gtggaaaagt tattcctgat cgttccacca
     3601 agccagtagt tttttctcca actctcatgt taacagatga agaaaaggct cgtattcatg
     3661 cagaaactgc tcttctaatg gaaaaaaaca aacaagaaaa agaacttcgg gaaaggcagc
     3721 aagaggaaca gaaagagaaa ctgaggaagg aagaacaaga acaaaaagcc aaaaagaaac
     3781 aagaagctga agaaaatgaa attacagaga agcaacaaaa agcaaaagaa gaaatggaga
     3841 agaaagaaag tgaacaggcc aagaaagaag ataaagaaac ctcagcaaag aggggcaaag
     3901 aaataacagg agtaaaaaga caaagtaaaa gtgaacatga aacttctgat gccaagaaat
     3961 ctgtagaaga tagggggaaa aggtgtccaa ccccagaaat acagaaaaag tcaacaggag
     4021 atgtgcccca tacatctgtg acaggggatt caggttcagg caagccattt aagattaaag
     4081 gacaaccaga aagtggaatt ctaaggacag gaacttttag agaggataca gacgataccg
     4141 aaagaaataa agctcaacga gaacctttga caagagcacg aagtgaagaa atggggagga
     4201 tcgtaccagg actgccttca ggctgggcca agtttcttga cccaatcact ggaacctttc
     4261 gttattatca ttcacccacc aacactgttc atatgtaccc accggaaatg gctccttcat
     4321 ctgcacctcc ttccacccct ccaactcata aagccaagcc acagattcct gctgagcggg
     4381 atagggaacc ttccaaactg aagcgctcct actcctcccc agatataacc caggctattc
     4441 aagaggaaga gaagaggaag ccaacagtaa ctccaacagt taatcgggaa aacaagccaa
     4501 catgttatcc taaagctgag atctcaaggc tttctgcttc tcagattcgg aacctcaatc
     4561 ctgtttttgg aggttctgga ccagctctta ctggacttcg taacttagga aatacttgtt
     4621 atatgaactc aatattgcag tgcctatgta acgctccaca tttggctgat tatttcaacc
     4681 gaaactgtta tcaggatgat attaacaggt caaatttgtt ggggcataaa ggtgaagtgg
     4741 cagaagaatt tggtataatc atgaaagccc tgtggacagg acagtataga tatatcagtc
     4801 caaaggactt taaaatcacc attgggaaga tcaatgacca gtttgcagga tacagtcagc
     4861 aagattcaca agaattgctt ctgttcctaa tggatggtct ccatgaagat ctaaataaag
     4921 ctgataatcg gaagagatat aaagaagaaa ataatgatca tctcgatgac tttaaagctg
     4981 cagaacatgc ctggcagaaa cacaagcagc tcaatgagtc tattattgtt gcactttttc
     5041 agggtcaatt caaatctaca gtacagtgcc tcacatgtca caaaaagtct aggacatttg
     5101 aggccttcat gtatttgtct ctaccactag catccacaag taaatgtaca ttacaggatt
     5161 gccttagatt attttccaaa gaagaaaaac tcacagataa caacagattt tactgcagtc
     5221 attgcagagc tcgacgggat tctctaaaaa agatagaaat ctggaagtta ccacctgtgc
     5281 ttttagtgca tctgaaacgt ttttcctacg atggcaggtg gaaacaaaaa ttacagacat
     5341 ctgtggactt cccgttagaa aatcttgact tgtcacagta tgttattggt ccaaagaaca
     5401 atttgaagaa atataatttg ttttctgttt caaatcacta cggtgggctg gatggaggcc
     5461 actacacagc ctattgtaaa aatgcagcaa gacaacggtg gtttaagttt gatgatcatg
     5521 aagtttctga tatctccgtt tcttctgtga aatcttcagc agcttatatc ctcttttata
     5581 cttcattggg accacgagta actgatgtag ccacataacc aactttcttg tacaaagtgg
     5641 ttcgataacg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     5701 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     5761 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     5821 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     5881 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     5941 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     6001 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     6061 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     6121 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     6181 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     6241 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     6301 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6361 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6421 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6481 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6541 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6601 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6661 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6721 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     6781 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgataa
     6841 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     6901 ttggcaagct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     6961 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     7021 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact
     7081 gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     7141 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     7201 catgggtaac agtttcttga agttggagaa caacattctg agggtaggag tcgaatatta
     7261 agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac tcctgaaata
     7321 gttcattatg gacagcgcag aaagagctgg ggagaattgt gaaattgtta tccgctcaca
     7381 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7441 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7501 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7561 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7621 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7681 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7741 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7801 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7861 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7921 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7981 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8041 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8101 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8161 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8221 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8281 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8341 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8401 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8461 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8521 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8581 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8641 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8701 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8761 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8821 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8881 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8941 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9001 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9061 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9121 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9181 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9241 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9301 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9361 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9421 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9481 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     9541 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     9601 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     9661 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     9721 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     9781 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     9841 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     9901 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga
     9961 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
    10021 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
    10081 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
    10141 gtccggcgta gaggcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc
    10201 tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat
    10261 aaaagatttt atttagtctc cagaaaaagg gggg
//
