LOCUS       sequence-127555-         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the Arabidopsis thaliana heat shock protein 18.2 terminator
            as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pTHsp18.2 (GB0035)
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(83..331)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     regulatory      370..379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      374..383
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(413..432)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(414..432)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(439..456)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(439..455)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(448..470)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcagcgtggg agctctccca tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt
       61 gggcgatgag tggtctcgag cgcttatctt taatcatatt ccatagtcca taccatagca
      121 catacagtag ttatatgctg cagaagagat ccaacaaaac attcacaatg gattatagaa
      181 acatttgttt attcattata atgagatctt acattcattt aatattagaa aaagccacaa
      241 attcataaca caacaagcca agaaaaaaac acaaacttaa gcacacaagc tttttatttg
      301 acacaccaaa tatttcatct tcatcttcat ataagccgag accactcatc gcccaatcga
      361 attcccgcgg ccgccatggc ggccgggagc atgcgacgtc gggcccaatt cgccctatag
      421 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      481 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      541 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
      601 ccctgtagcg gcgcattaag cgcggcgggt gtgg
//
