LOCUS       sequence-127565-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-STRHISNDHFR
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             29..51
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             68..91
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             101..118
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             146..166
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(745..763)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attggaattc gctagctcta gaaataattt tgtttaactt taagaaggag atatacatat
       61 ggcaagctgg agccacccgc agttcgagaa aggatctagc catcatcatc atcatcacag
      121 cagcggatct ggtggtggtg gtggtgaaaa cctgtacttc cagggatcca tcagtctgat
      181 tgcggcgcta gcggtagatc gcgttatcgg catggaaaac gccatgccat ggaacctgcc
      241 tgccgatctc gcctggttta aacgcaacac cttaaataaa cccgtgatta tggggcgcca
      301 tacctgggaa tcaatcggta ggcctttgcc cggccgcaaa aatattatcc tcagcagtca
      361 acccgggacc gatgatcggg ttacctgggt taaatcggtc gacgaagcca tcgcggcgtg
      421 tggtgacgta ccagaaatca tggtgattgg cggcggacgc gtttatgaac agttcttgcc
      481 aaaagcgcaa aaactgtatc tgacgcatat cgatgcagaa gtggaaggcg acacccattt
      541 tccggattac gagccggatg actgggaatc ggtattcagc gaattccacg atgctgatgc
      601 gcagaactcg catagctatt gtttcgaaat cctcgagcgt cgtgccggct aatgtacaag
      661 atccagatct aagcttggta ccacgcgtgc gcgctgatcc ggctgctaac aaagcccgaa
      721 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      781 ctaaacgggt cttgaggggt t
//
