LOCUS       sequence-127566-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-DHFRHIS
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..75)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             572..594
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(661..680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(662..680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat cagcgcgcac gcgtggtacc aagcttagat ctggatcttg tacattagtg
       61 gtggtggtgg tggtggccgg cacgacgctc gaggatttcg aaacaatagc tatgcgagtt
      121 ctgcgcatca gcatcgtgga attcgctgaa taccgattcc cagtcatccg gctcgtaatc
      181 cggaaaatgg gtgtcgcctt ccacttctgc atcgatatgc gtcagataca gtttttgcgc
      241 ttttggcaag aactgttcat aaacgcgtcc gccgccaatc accatgattt ctggtacgtc
      301 accacacgcc gcgatggctt cgtcgaccga tttaacccag gtaacccgat catcggtccc
      361 gggttgactg ctgaggataa tatttttgcg gccgggcaaa ggcctaccga ttgattccca
      421 ggtatggcgc cccataatca cgggtttatt taaggtgttg cgtttaaacc aggcgagatc
      481 ggcaggcagg ttccatggca tggcgttttc catgccgata acgcgatcta ccgctagcgc
      541 cgcaatcaga ctgatggatc ccatatgtat atctccttct taaagttaaa caaaattatt
      601 tctagagcta gcgaattcca attggagctc tccggagccg gagggaaacc gttgtggtct
      661 ccctatagtg agtcgtatta atttcgcggg atcgatgcat ggtgccaaca cctacatctg
      721 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
      781 ccgccagttg t
//
