LOCUS       sequence-12757-e       16771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16771)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 16771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2008 Nov 1. 323(1):76-87."
COMMENT     Sequence Label: pCG111
FEATURES             Location/Qualifiers
     source          1..16771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     668..684
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2641..2652)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(9956..9972)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10026..10054)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10802..10822
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             10908..10928
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             10941..10985
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    10998..11022
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11335..11359)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(14580..14598)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14619..14635)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14619..14635)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14632..14654)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14643..14659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14667..14673,14674..14691,14692..14697))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(14850..14867)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15021..15609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(15780..16571,16572..16640))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16403..16422
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16641..16745)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc
      661 cccccctcga ggtcgacggt atcgataagc ttcagtaaaa gaagtagaat tttatagttt
      721 tttttctgtt tgaaaaattc tccccatcaa tgttctttca aataaataca tcactaatgc
      781 aaagtattct ataacctcat atctaaattc ttcaaaatct taacatatct tatcattgct
      841 ttaagtcaaa cgtaacatta aaaaaaatgt tttggaaaat gtgtcaagtc tctcaaaatt
      901 cagtttttta aaccactcct atagtcctat agtcctatag ttacccatga aatccttata
      961 tattactgta aaatgtttca aaaaccattg gcaaattgcc agaactgaaa atttccggca
     1021 aattggggaa ccggcaaatt gccaatttgc tgaatttgcc ggaaaaacgg taattgccga
     1081 aagtttttga cacgaaaatg gcaaattgtg gttttaaaat tttttttttt ggaaatttca
     1141 gaatttcaat tttaatcggc aaaactgtag gcatcctaag aatgttccta catctatttt
     1201 gaaaagtaag cgaattctat gaaaatgtct aaagaaaatg gggaaacaat ttcaaaaagg
     1261 cacagtttca agtgtttccg aattatacta aatccctcta aaaacttccg gcaaattgat
     1321 atccgtaaaa gagcaaatcc gcatttttgc cgaaaattaa aatttccgac aaatcggcaa
     1381 accggcaatt tggcgaaatt tgccggaagg caattgccgc ccacccctgt tccagaggtt
     1441 caaactggta gcaaagctca aaatttctca aattctccaa tttttttttg aattttggca
     1501 gtgtaccaaa atgacattca gtcatattgg tttattatag atttatttag ataaaatcct
     1561 aaatgattct acctttaaag atgcccactt taaaagtaat gactcaaact tcaaattgct
     1621 ctaagattct attgaattac catcttttcc tctcattttc tctcactgtc tatttcatca
     1681 caaattcatc cctctctccc tctctttctc tctccctctc tctctttctc tttgctcatc
     1741 atctgtcatt ttgtccgttc ctctctctgc gccctcagcg ttccccacac tctctcgctt
     1801 ctcttttcct agacgtcttc ttttttcatc ttcttcagcc ctttttcgcc attttccatc
     1861 tctgtcaatc attacggacg acccccatta atttttggat gtgctctgcg agcaagagca
     1921 cacggaactc atttattttt tgttggaatt tctttttcat tttagttagt taaaaggtta
     1981 ttcagtgcaa aaattgattt tttgctcttt tggtacatta gaggggtttg aaagttttcc
     2041 ataaaaaggg tttccggagt aaaaaatcta aaaattttta caaacatttc caaaaatttt
     2101 gggaacatat cttttcaaaa actcaaaaaa aaacgcaaac atcataaatt taatcaatta
     2161 attggatgcg ttcaaatatt ttaaaaatat ttttgaattg catatttctt tactcataaa
     2221 aaaattatta atgtttaagt aaaaaaaata tttcaggatt ttaaacaaaa actgtaaatc
     2281 ccataaaaca gtgttatttt ttagttattt ttagtaatta tttgtgaatt aaaaacacat
     2341 tttttttcaa aatattcctt gaataaataa aattgaaagt gaattcaaaa tatttcacca
     2401 attttacaaa atttgaaaat ttgaaaaaat attttctacc aaattgtgct caaaaaatat
     2461 tattaagttc ccaggaaatt tatgatgaaa acttggaata tttttggtaa aattttctca
     2521 aaaaattgaa agttgtcaaa aacacactaa cactatacct cgcggcatag aaaaaactgg
     2581 tggccgaatt ttttaaacca atttaaatta aatatatttt tgcactttgt acaaaaaaat
     2641 ttgaagttcc caggaaattc atacggaaat attttccaga acttttataa tttttatttt
     2701 aattctcaaa atttacaata aaaacttgga aacctgaaat ttgcttaaag aataacccta
     2761 tcccccacgg cctagaaaat actggtggcc gaattttttc cgcagccaca ccacctctaa
     2821 tctccagagc actctccaaa attcccataa tccccaaaat tttcagctac aacagcatat
     2881 atgaaggcag agcaacaaca acaatcgatc gcacccggct cggcaacctt cccgtctcag
     2941 gtgaggaaga cgcagagaaa tgagagagag agcgtgttta tgcacgcagt atactaactc
     3001 ctgatatcca atttcatata tcgatatgct tgctttcttt tcttctacgt tgaaattgtt
     3061 tgaacttttc tcaaaaaacg cggattttag agagacttag agttgctcca aataaattta
     3121 atttgcaatt ttgcacttac tattagaatc tacttttata gcgctaaaat gaccgaataa
     3181 cacagtagat tttaaaagaa aattttaaaa attatttttt tgtgcagtca aatttcgcta
     3241 gtttttgcca ctcatttttg aacatttgta tatgtgcctt tcggtggttc aatcaggggt
     3301 ggcacgtggt gtcagagtgt tccatttcgg tttgatctac gttgatctac aaaaaatgcg
     3361 ggagaagaga cgcagagttc tcaactgatt tcacatggtt atgaaccagg ggtgttcggc
     3421 aaatttgccg tttgccgagc tgggcaaatt ttgagatttg ccgcacaccc cttttatgaa
     3481 cgtgctgacg tcacatcttt ttggacaaag aattcccgca ttttttgtat atcaaaccgt
     3541 gatgggacag cctggcacca cgtggggttg gcggcaaatg attttttccg acaaatcggc
     3601 aaattggcaa attgccggaa tttaaagttt ctggcaaatt gttgatttgc cgaaaaaaaa
     3661 tttcaaaaag gcacagtttt aagtgtttcc gtcttataaa atatttctct aaaaatttcc
     3721 ggaaaacgga aaattgccgg aaatacaaag tttcgtcaaa tcggcaaacc tgcaatttgc
     3781 cgaacggcaa ctgccgccaa cacctggttc ccaaaaaagc gtgcactgtt tgaatattaa
     3841 aaaaaaaatt cacacaaaaa taataaattg aaataggcta aattcacatc tatgtacaaa
     3901 aattgcatgc cagcaccggt caattttctc aattactttt tgtctatttt tgccgaacat
     3961 aatttgctgt ttccttggca gaatacagaa aactcttttt tttttcagat gccccgaccc
     4021 ccaccggtaa ccgaacaggc tataaccacc gaggcggagc ttctcgcgaa aaatcaaatt
     4081 acaccaaatg atgtgctagc tcttccagga atcactcaag gtaagtgaat atttgctcta
     4141 ttgataaacg cgccgtgtac tccacgtgga caaacatatc aaattttcag gattcctatg
     4201 ctccccatcg gcaaacgtct ataacattga gttcaccaaa ttccaaatcc gtgacctcga
     4261 cacggaacac gtgctcttcg aaattgcaaa accggaaaat gagacggaag agaatctgca
     4321 ggcgcaagcc gaatcggcaa gatatgtccg atatcgattt gcgccgaatt ttctgaaatt
     4381 aaagacggtc ggcgcgacgg tcgagttcaa ggtcggcgat gtgccgatca cacattttcg
     4441 aatgattgaa cggcactttt tcaaggatcg tttattgaaa tgctttgatt ttgaatttgg
     4501 attctgtatg ccaaattcga gaaacaactg tgaacatatc tatgaatttc cacaactttc
     4561 acaacaactc agtgagtttg aagcgggaaa aattatttga gctcattttg tcaatagagc
     4621 gaattttcta ggtttttaaa aataaaataa atattgacaa aaagctgccc tacagtagtt
     4681 caaaatgtga aaacttggag gaaattcgag ttttctctga gaagtttttg gcgggtaatt
     4741 caaattttct gagaaaaaaa ttagccgcga gaattcaaat tttctgagaa aattatttga
     4801 cgggaaattc aaaatttctg gaaatgtatt gaacatttga gaaattttgt taaaaactac
     4861 attaaaccga taaaaaagtt cgcaaatttt ttttaagtgc tcatggtgca tcgaagagaa
     4921 gaattcttgc caaaactagc ccatgaaatt aaaaattatc gcggaaaaaa actgactaat
     4981 tggaaagtaa aatgaaattt gcaaaaaaaa aatatgtttt tttgttgtaa aattaaaaaa
     5041 aaaactgtat attttcagtg gacgacatga tcaataatcc aaacgagacc cgctccgata
     5101 gcttctattt tgtggagaat aagctcgtaa tgcacaataa ggccgactac tcgtatgatg
     5161 cataatttcc cgccgatgga ataaaactaa taatttttct cgcaatttat agaaatcatg
     5221 tgattttcct accgctaaat ttaaaatttc ctaatttttc actatatttc tcacaaaatg
     5281 cattccgaaa actctcattt ttcagctttt caatacaaat cccaataaat gatttttcaa
     5341 atataatgtt ctcaattgat atgttttatt tgaatgttgg aaaaaaccat aaaattaaaa
     5401 aaaacttgaa ttattcaaaa ataattcggc ccttttacaa aaaattatca taaaaattaa
     5461 atttggaaaa tctgtgattt gtcgctttgc cggaagtttc caattccggc aactaaccga
     5521 tttgccgatt agctagaaat ttcaattccg acaatgtgcc gctttgccga tttgccggaa
     5581 atttccaatt ccggcaatta acggacttgc cgattagcta gaaatttaac tttagataaa
     5641 acttctcaac aaaatcaaat tggaaagtca gcgatttgcc gctttgccgg aagttttcag
     5701 ttccggcatc taaccgattt gccgatttgc caaaaatgtc aattccgaaa aactgccgat
     5761 ttgccgcttt gtcggaaatt tcaattccga caaattgccg atttgccgat ttgccgaaca
     5821 ttccaattcc gaccatttgt cgatttgccg gaagttacca attccggcaa ctaacggatt
     5881 tgccgatttg ccggaaaaaa attgtttgtc gccaaccctg atttaaatga ctaatcatgt
     5941 tgcaaaagat aactgcttgc atcaaaaatc cgatcattgg cattcccaac atcttcttca
     6001 tccaaaacac cacccttttc ttccactaat catgaattcc cgaccaattg ttatcagtag
     6061 gagaagcctc cttttatctc attatcagca attgccttct tcttcttctt ctttgaatac
     6121 catttgaatg gagatatcat ttacataaat ctgccgccga gattgtgaag caggaggaaa
     6181 agctgaagat actgatatta tatgtatatt tcattattct ttttccacaa aattttccga
     6241 catttaattc cacttcactt tcatcgtctc tttttccccc attgtcgccc ccactcattt
     6301 gtcttcttct tcaaatacgg gtcaatcact ttccgttttt tattcatttt ttgttatgtt
     6361 cggcttaaca aatttgaagt attgttaaaa ttctctagag cggccgccac cgcggtggcg
     6421 gccgcgcgga agatctggaa gtaaaatatt gtaatatttt ccctaaattt cacttaaaaa
     6481 taaggatttt tgcaacgtaa aacctcaaaa attcgctgaa attgacgatt tttgttcaaa
     6541 attttgaaaa acagggttaa ttttagctga aaatcttcaa ttttgccaga aattggcttt
     6601 ttttccgtcg ccacacggag aacttgccaa aaatgaagac ttttagccaa tttagccctg
     6661 aaaattcgag ttttgggcga tttttggtct gagaagccgt tttcgcagta tattatattt
     6721 tctcgttaaa tttcatttta aaataaacat tttgaacgta taaccccaaa aattcgctgc
     6781 gcaatggatg atttttgtcc aaagttttga taattttagc taaaaacctc gaattttggc
     6841 aatttttcgt gccgcatcgg ccgaaagttg actttttttg gaattatcat aatttaatgc
     6901 acattttggt agtttatctc acataatctg tctgcaggcg ggaaaaattt gagataaact
     6961 actaaaagat gcaaaacaag gatgataatt ccagaaaatc aacttcggac cgtctcgaca
     7021 cggaaaattg agcgaaattg gagttttaaa atgatttagc cctgaaaatt cgagaaattt
     7081 aggcggtttt gggtctgaaa aggcgttttc gcagtatttt ctcgctaaat ttcatttatt
     7141 atcacataat atcagtactt aggcgaatat cttgtagaaa tgttggtttt gttcgataat
     7201 tctctacttt tagagtcgat tcacgagaaa atgttgcacc atcttacttt tttcccttgt
     7261 aaaacataga ttcttctgta caatgaaaga gaacacaatt tcttcgtgaa gcaactcaaa
     7321 aagtagagaa aattcgaaca aaacccaaca tttctacaag atattcgtct aaatagtgat
     7381 ataatgtgtt aataagcata ttttaaatga aatttagcga aaaaactgcg aaaatttgtc
     7441 aaaaaagcga atttccatcc gatcagcaga tctctaaaag ttacataaaa ttgaaagttt
     7501 tgtggggaaa ttgttttaaa acacgcctat ggtaatacga gaacataaaa ttctgagaat
     7561 gcgtattaca caacatattc gacgcgcaaa atataactat atctcgtagc gaaaactaca
     7621 gtaatcctta aatgactact gtangcgatt gtgtcgattt acgggctcga ttctcggaaa
     7681 aaaaaatcat ttttcaaatt ttgacagcga tattcaattt ttcttcgttt tttcgtatnt
     7741 tctcagtcat ttttgtgctt atttttaata ttttattcat taataaattn aatttaattg
     7801 aaaaacgtcc agacgtattt ttacatttcc cgtctatttc cgtaataggc ggttaataac
     7861 gtagaaatac gtctgtatga gtttactcat caaaatgaca cgaatattgg acgtttttaa
     7921 atcaaattaa tttattaata ggtaaaatat taaaaaataa acacaaaaat gacaaaggaa
     7981 taggaaaaca cgaagagaaa ttgagtatcg ctgtncaaaa ttcgaaaaac gtggattttt
     8041 ccaagaatcg agcccgtaaa tcgacacatt cgctacagta gtcatttaag gattattgta
     8101 gttttcgcta cgagatactt tgcgcgccaa aaacgtggtg aaatacgcat tctcagaatt
     8161 ttatgttccc gtaataccac aatgaccgaa tatcataata aaaaaattca aaaacaattt
     8221 ctagatttta tatgattttc tgaaaattga aaaaatctca gttttcacct aattatattt
     8281 gaactacagc caattgaact cgttcgttgg agcgcgcttg cattattttc attaattaat
     8341 tttattaatc ctcattattt cactgatttt cttcattttt gaggtttttt ttatcgggaa
     8401 atgaaagaaa tatacaagaa aaatgcaaaa tgtttattaa aaactgaaat tagtatttct
     8461 cggagtttta ggcattttca gttacttttt aataaagatt ttgcattttt cttgtatatt
     8521 tctttcattt tccgattaaa aaaccccaaa aatgaagaaa atcagtgaaa taatgaaaat
     8581 taattcaaat aactaatgaa aataatgcaa gcgcgctcca accaacgagt tcaattggcg
     8641 gtaattcaaa tataattagg tgaaaactga gattttttca attttcaaaa aatcatataa
     8701 catctagaca atttttttga atttttttat catgatattc ggtcattgtg tccccatagg
     8761 catgttttaa agcaatttcc acctttaaat aaaatcgaga aaaaatggcg tcaaaagaca
     8821 tatgtaaaag ctgtttctct ttgtatattc acacagaata tagcccgatt ttggaggtga
     8881 aaaaccggtt tttttgtatt ctttacgcca cataaagtga tatggaagag aaaatcgaca
     8941 tatttatgta cttggagtac gagtacattg gagaaaaagg gtgaactact ggaaaatctg
     9001 ggaaatttca gaaaaaaatt ccgaaaaatc ttttttgctg aaaaaaaaat ttgttcgaaa
     9061 tttgggaaat actggaaaat tttcatcaaa attaaagcga tttttaacgt ttttgcttgg
     9121 gagatactat ttgtaatttt aatgtttggt gaggacaaca gaaatgacct tcagcgtacg
     9181 gaaaacgatt aaaagacaca tttgaatcga aaatagcgtg aaaaacatga aaatatcgga
     9241 aaaatagctt taaaattgga tttgaaagca aaaaatgtat caaaaatcga gaaaaatgct
     9301 tgaaaaagtt gaaaaactca aacaaaaaat caaaaaaggt ttttttaaaa atctgtgagt
     9361 atcgcaacga aagcacaact tgaggcatgc gcctttaaac caaccgtaac caaattcgga
     9421 ggcaaaaaaa gatttctcgc tgttttttca gttttaaaat cacttttcac gtttcttttc
     9481 gcagtaattc gtttgcaaaa catctgaaaa aattttattt tataagaaat tatataaaaa
     9541 tcgctataaa ttgagttttt gcccccaaat tcagcacgga gcacttctca attaaatata
     9601 attttatttt ttaacattga aagctttcat aaaattgagc tttgtttgta aataatcagt
     9661 gaaaaaacac agaaatcttc aaaaaataaa gaaaaagttt ttaaaaatct gtgagtcccg
     9721 caacgaaaat aaaactttag gcatgcgcct ttaaaccaac cgtaaccaaa ttcggcggca
     9781 aaaatggatt tctcgccgtt ttttcagttt tacaatcact ttttaggttt ctttttgcag
     9841 tatttcgttt cccaaacaat taaaaatcaa attttctttt ccagatggct caaacaaagc
     9901 cgattgccga gcaaatggga tcccccgggc tgcaggaatt cgatatcaag cttatcgata
     9961 ccgtcgacct cgagggggcg gtaccggtag aaaaaatgag taaaggagaa gaacttttca
    10021 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
    10081 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
    10141 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
    10201 attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct
    10261 cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
    10321 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
    10381 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
    10441 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
    10501 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
    10561 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
    10621 ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
    10681 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
    10741 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
    10801 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
    10861 aatgcccggg ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt
    10921 ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
    10981 acagcggagg tggaggtaca agtttgtaca aaaaagcagg cttaatggcc accgaaaaga
    11041 aagaagtgac actgtctgct acagtcgaag agccactcga tttgctccgt ctcagtttgg
    11101 atgagagagt ttatgtcaaa atgagaaacg acagagagtt gcgcggtcgt ctcagagctt
    11161 tcgatcaaca tttgaacatg gttttatcgg aggttgaaga gacaatcacc acccgtgaag
    11221 tcgacgaaga cacattcgaa gagatctaca aacaaacaaa acgtgttgtt ccaatgcttt
    11281 tcgttcgtgg tgattctgtc attctcgtgt ctccaccgat tcgtgcatcc taaaacccag
    11341 ctttcttgta caaagtggtt gatctagtgg atcctaattt tgccgtattt tccatatttt
    11401 gttttgtata tttatccact caccccctct ctttgtcctg tgaatgaact tgtgccaaaa
    11461 aagccaaaaa taatttactt tttttaaaga catattaaaa cttgaaaact tggaacaata
    11521 cggtgcgaaa gcgaaacaga gacagaaaaa tgaacaaaga gatccgtgaa aagtgaacga
    11581 tgatttaggc ttcttgttgg gcggcgtgga tgagagtgat gttgtctcct ttgagaagaa
    11641 ttctgccgat cttgttgcgt cccttcgtct tcatgttcac ctcctcggct tcgtcgaaca
    11701 cgacattcat gaactcgtcg aatccgatga tgtagccctc gagacggtgg gtgacatcct
    11761 cgtacaacca gatttggacc cgagtgcggt tttggagata tctgaaattt gggaaattgt
    11821 tttctactga ttagtgaaaa aatattacct gaagatgagg ttcacaggct gaaccatcac
    11881 tttgttgagc tttctcgtcg acatttttgc gggatgatct gtaaaaatca atatattttt
    11941 tgaaagacac aaatttttaa gataaatatc aagaaaactc gattttagca gctatttaag
    12001 ctcaaaaact acaatttatg gctttaaaac ataatttttc acattttaca taaatttcaa
    12061 cgaaaaaaaa tgccaaaaaa cttcaaattt gagcagaaat cggctatttt ctcgacaaaa
    12121 ataatttcga aaattcgttc aaaaaagatg agtacgcaag caccgtgacg cacagttgcg
    12181 gactttgtaa accgtacccc tgcataaaaa ggcggaattt tgaatttaat aaaatgcagt
    12241 ttagaacaaa gaaaagcacg gagagaattg tgataactaa ttatttgtat ttaattttaa
    12301 atactacata aaactgaaga ttggaacgag ttaaagctgg tatttgcggt ttatagcccc
    12361 aaaagttaag gcttgttagg caattatcga taaaaacaac gagaaattcg tgaaatcggc
    12421 aaaaatttat agaaaacgtt tgaattaaat taaatttacg cttttgccgc caacacctat
    12481 cgagacccta gttatagggg cggagcgtca tgggagcgcg cttgcaactc gatttcaaaa
    12541 agtttcgctg tttgtacaac attctcagtt gttcttaatc agttaaataa gtttcaaaat
    12601 tttnncaaaa tcataaaaca cagttaatgc taaattaaag tctaaaaana tcaggtgata
    12661 aggggtttgg acgaccaaaa agcgaccttc gctcgaaaat tgataaacta actacacttt
    12721 ttccggaaaa atgatggaaa aatcctgatt ttcttcgttg aatcaatttt tgtttcattt
    12781 ttaacacttt cacatganna atatttcagt taaaatgctc aagctaattg ggtttcttat
    12841 aattttgggt ctcgcaacgg ccgacaagtg ctcagattgt ttggctcagg ggaaacagtg
    12901 agttggaagc ttacaattct gagaattcgt attgcgcagc ttatttgacg cgcaaaatat
    12961 gaaatatcac gtagcgaaaa ctacagtaat tctttaaata agactactgt agtgcctatg
    13021 tcgatttact gcctcaactt tcttaatcaa gctcgtaaat cgacaccagc tctacagtag
    13081 tcatagtttt cgctacgaga tattttgcgc gtcaaatatg gtgtgcaata cgcattttca
    13141 gaattttatg ttctcgtaat ataccttttt tcagctggtg tgttactgta aatgcatgtg
    13201 gatttacgcc atgtgtcact acaattacag tgagtttttt tgaagaaaaa aaaattaccc
    13261 atttgatcgc caattccatt tttcaaaaat caataaaaaa aaaaaatttc ggcgatcaga
    13321 aacgttacag aaagccctaa actgcccgga actaccagat aaagcccacg cttacgatga
    13381 ttcatttatg aggaataagg tattgatcac acattctgct gcctacaata tggacccaca
    13441 aaagtgtttt acgtaggttc accttctaga taaaccttaa aaatcgatac tttcagtagt
    13501 aaaatgcctt cgatgaaagt ttccaaaaca gttaatgtca attgtgatag atatgggcca
    13561 agtgagttgt tttattagcc cacggatttc tggcttccct cataaattga aaaggaagag
    13621 attgccgaac tagaccattt tggctcggcg cgttgcgtgt cacgtcgcgg ctcgattgta
    13681 aaactaaatg tattggtccg tgtggagtac acgactttcc ctttccggca ggcgattgtc
    13741 aatggagcgc gaaaaattca atgaggaagg ccagaacccc gtgtctgtag ttttctcaaa
    13801 aagtggcaaa aactcaataa ttgccaattt ttgactgcca ataaaaattt cactaaattt
    13861 attaaattta ttttaagttt tattgaaatt tttttaaaat ttccccactg gcgctaaaaa
    13921 atccaatttt cagaagcaat gtgcttcagc ttcacggcca tagatactgt ccaaaaagtg
    13981 attgcaatga cgtttagagg gacagaagga gcagctcaat tgtctgaaga aattttggat
    14041 ttcttccagt aagttcggga aatggcaatt taaagtttaa ttggattttt gtattttttc
    14101 ttctcacgac tacagttttg cacattttta tgttataaaa aaccaaaaaa aaattttttt
    14161 gccttttttc tcagcttacc acaaaacaat ttcgaccaaa aataaattga aaaaattaaa
    14221 aaattttttg cacggttttc ggactcaaaa aaagctgaat gttcctggaa ctatttgtaa
    14281 aattcaaaat tcaaaatttt cacgaattct ctaaaaggtt ccagcaaatt ccacaacttt
    14341 cttgaatttt tcaaaaaagt tcttgagcat tcaacaatta tttttggaat tttccaaagg
    14401 ttacaggacg ttccaatttt tcaaaaaaat ttctcgcaaa aaatgttttc tcagaaaatt
    14461 tgaatttccc tccacataaa agattgttct caaaaaattt gcaaacacac attttcagcg
    14521 gaaaaaaggc gttcttcgac tctggaagtg tattcgaata tggtacccag cttttgttcc
    14581 ctttagtgag ggttaattcc gagcttggcg taatcatggt catagctgtt tcctgtgtga
    14641 aattgttatc cgctcacaat tccacacaac ataggagccg gaagcataaa gtgtaaagcc
    14701 tggggtgcct aatgagtgag gtaactcaca ttaattgcgt tgcgctcact gcccgctttc
    14761 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    14821 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    14881 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    14941 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    15001 aaggccgcgt tgctggcgtt tttccatagg ctcggccccc ctgacgagca tcacaaaaat
    15061 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgttcccc
    15121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    15181 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
    15241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    15301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    15361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    15421 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    15481 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    15541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    15601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    15661 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    15721 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    15781 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    15841 gttgcctgac tgcccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    15901 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    15961 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    16021 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    16081 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    16141 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg aaaaaaagcg
    16201 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    16261 atgcttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    16321 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    16381 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    16441 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    16501 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    16561 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    16621 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    16681 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    16741 ccgcgcacat ttccccgaaa agtgccacct g
//
