LOCUS       sequence-127570-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-DHFRCYD
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(630..648)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      644..691
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaattcaata attttgttta actttaagaa ggagatatac atatgggatc catcagtctg
       61 attgcggcgc tagcggtaga tcgcgttatc ggcatggaaa acgccatgcc atggaacctg
      121 cctgccgatc tcgcctggtt taaacgcaac accttaaata aacccgtgat tatggggcgc
      181 catacctggg aatcaatcgg taggcctttg cccggccgca aaaatattat cctcagcagt
      241 caacccggga ccgatgatcg ggttacctgg gttaaatcgg tcgacgaagc catcgcggcg
      301 tgtggtgacg taccagaaat catggtgatt ggcggcggac gcgtttatga acagttcttg
      361 ccaaaagcgc aaaaactgta tctgacgcat atcgatgcag aagtggaagg cgacacccat
      421 tttccggatt acgagccgga tgactgggaa tcggtattca gcgaattcca cgatgctgat
      481 gcgcagaact cgcatagcta ttgtttcgaa atcctcgagc gtcgtgccgg caccgaattt
      541 tgcgcttaat gtacaaagct tggtaccacg cgtgcgcgct gatccggctg ctaacaaagc
      601 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      661 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat cgggattagc
      721 ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc
      781 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaa
//
