LOCUS       sequence-127572-         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses p53 tagged with mKate2 and split C-term mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Gaglia G, Lahav G
  TITLE     Constant rate of p53 tetramerization in response to DNA damage 
            controls the p53 response.
  JOURNAL   Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168.
  PUBMED    25344068
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-10-24"; volume: "10:753. doi"; pages: " 
            10.15252/msb.20145168"
COMMENT     Sequence Label: pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..26
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(73..94)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attatcaact atgtataata aagttgatta cttgtacagc tcgtccatgc cgagagtgat
       61 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      121 gctcagcttg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      181 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg
      241 gcggatcttg aagttggcct tgatgccgtt cttggctccc ccacctccac ctccacctcc
      301 tcctctgtgc cccagtttgc tagggaggtc gcagtatctg gccacagcca cctcgtgctg
      361 ctcgacgtag gtctctttgt cggcctcctt gattctttcc agtcttctgt ccacatagta
      421 gacgccgggc atcttgaggt tcttagcggg tttcttggat ctgtatgtgg tcttcaagtt
      481 gcagatcagg tggcccccgc ccacgagctt cagggccatg tcggctctgc cttccaggcc
      541 gccgtcagcg gggtacaggg tctcggtgga ggcctcccag ccgagtgttt tcttctgcat
      601 cacagggccg ttggatggga agttcacccc tctgatcttg
//
