LOCUS       sequence-127582-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mutated p53-L344P tagged with mKate2 and split N-term 
            mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Gaglia G, Lahav G
  TITLE     Constant rate of p53 tetramerization in response to DNA damage 
            controls the p53 response.
  JOURNAL   Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168.
  PUBMED    25344068
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-10-24"; volume: "10:753. doi"; pages: " 
            10.15252/msb.20145168"
COMMENT     Sequence Label: pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(628..652)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcgatggtct ggcccctcct cagcatctta tccgagtgga gggaaatttg cgtgtggagt
       61 atttggatga cagaaacact tttcgacata gtgtggtggt gccctatgag ccgcctgagg
      121 ttggctctga ctgtaccacc atccactaca actacatgtg taacagttcc tgcatgggcg
      181 gcatgaaccg gaggcccatc ctcaccatca tcacactgga ggatagctct ggaaacctac
      241 tgggacggaa cagctttgag gtgcgtgttt gtgcctgtcc tgggagagac cggcgcacag
      301 aggaagagaa tctccgcaag aaaggggagc ctcaccacga gctgccccca gggagcacta
      361 agcgagcact gcccaacaac accagctcct ctccccagcc aaagaagaaa ccactggatg
      421 gagaatattt cacccttcag atccgtgggc gtgagcgctt cgagatgttc cgagagccga
      481 atgaggcctt ggaactcaag gatgcccagg ctgggaagga gccagggggg agcagggctc
      541 actccagcca cctgaagtcc aaaaagggtc agtctacctc ccgccataaa aaactcatgt
      601 tcaagacaga agggcctgac tcagacgacc cagctttctt gtacaaagtg ggaatggtgt
      661 ctgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc
      721 accacttcaa gtgcacatcc gagggcgaag gcaagcc
//
