LOCUS       sequence-127584-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mutated p53-L344P tagged with mKate2 and split N-term 
            mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Gaglia G, Lahav G
  TITLE     Constant rate of p53 tetramerization in response to DNA damage 
            controls the p53 response.
  JOURNAL   Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168.
  PUBMED    25344068
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-10-24"; volume: "10:753. doi"; pages: " 
            10.15252/msb.20145168"
COMMENT     Sequence Label: pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..48
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(join(62..520,521..523,524..526))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     201..220
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     460..481
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tccggatctc gacggccagt gaattatcaa ctatgtataa taaagttgat tactgcttgt
       61 cggcggtgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
      121 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
      181 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
      241 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
      301 ggtagcgggc gaagcactgc aggccgtagc ccagggtggt cacgagggtg ggccagggca
      361 cgggcagctt gccggtggtg cagatcagct tcagggtcag cttgccgtag gtggcatcgc
      421 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
      481 ggatgggcac caccccggtg aacagctcct cgcccttgct caccattccc actttacctc
      541 cacctccacc tcctcctctg tgccccagtt tgctagggag gtcgcagtat ctggccacag
      601 ccacctcgtg ctgctcgacg taggtctctt tgtcggcctc cttgattctt tccagtcttc
      661 tgtccacata gtagacgccg ggcatcttga ggttcttagc gggtttcttg gatctgtatg
      721 tggtcttcaa
//
