LOCUS       sequence-127589-         303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses p53-L344P mutant tagged with mCerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Gaglia G, Guan Y, Shah JV, Lahav G
  TITLE     Activation and control of p53 tetramerization in individual living 
            cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15497-501. doi: 
            10.1073/pnas.1311126110. Epub 2013 Sep 4.
  PUBMED    24006363
REFERENCE   2  (bases 1 to 303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1311126110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-09-17- 17"; volume: "110"; issue: "38"; pages: 
            "15497-501"
COMMENT     Sequence Label: pRRLNeo-pEF1a-p53ashL344P-mCerulean
FEATURES             Location/Qualifiers
     source          1..303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..27
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(31..282)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLXPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aattatcaac tatgtataat aaagttgtta cttgtacagc tcgtccatgc cgagagtgat
       61 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      121 gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc ancagcacgg ggccgtcgcc
      181 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
      241 gcggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga tatagacgtt
      301 gtc
//
