LOCUS       sequence-12759-e       10925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2008 Nov 1. 323(1):76-87."
COMMENT     Sequence Label: pJH4.52
FEATURES             Location/Qualifiers
     source          1..10925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4198..4214)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4199..4215)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4268..4296)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5044..5064
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(8734..8752)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8773..8789)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8773..8789)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8786..8808)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8797..8813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8821..8827,8828..8845,8846..8851))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(9004..9021)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9175..9763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9934..10725,10726..10794))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10557..10576
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10795..10899)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctacgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaggggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctccaccgcg
      661 gtggcggccg cgcggaagat ctggaagtaa aatattgtaa tattttccct aaatttcact
      721 taaaaataag gatttttgca acgtaaaacc tcaaaaattc gctgaaattg acgatttttg
      781 ttcaaaattt tgaaaaacag ggttaatttt agctgaaaat cttcaatttt gccagaaatt
      841 ggcttttttt ccgtcgccac acggagaact tgccaaaaat gaagactttt agccaattta
      901 gccctgaaaa ttcgagtttt gggcgatttt tggtctgaga agccgttttc gcagtatatt
      961 atattttctc gttaaatttc attttaaaat aaacattttg aacgtataac cccaaaaatt
     1021 cgctgcgcaa tggatgattt ttgtccaaag ttttgataat tttagctaaa aacctcgaat
     1081 tttggcaatt tttcgtgccg catcggccga aagttgactt tttttggaat tatcataatt
     1141 taatgcacat tttggtagtt tatctcacat aatctgtctg caggcgggaa aaatttgaga
     1201 taaactacta aaagatgcaa aacaaggatg ataattccag aaaatcaact tcggaccgtc
     1261 tcgacacgga aaattgagcg aaattggagt tttaaaatga tttagccctg aaaattcgag
     1321 aaatttaggc ggttttgggt ctgaaaaggc gttttcgcag tattttctcg ctaaatttca
     1381 tttattatca cataatatca gtacttaggc gaatatcttg tagaaatgtt ggttttgttc
     1441 gataattctc tacttttaga gtcgattcac gagaaaatgt tgcaccatct tacttttttc
     1501 ccttgtaaaa catagattct tctgtacaat gaaagagaac acaatttctt cgtgaagcaa
     1561 ctcaaaaagt agagaaaatt cgaacaaaac ccaacatttc tacaagatat tcgtctaaat
     1621 agtgatataa tgtgttaata agcatatttt aaatgaaatt tagcgaaaaa actgcgaaaa
     1681 tttgtcaaaa aagcgaattt ccatccgatc agcagatctc taaaagttac ataaaattga
     1741 aagttttgtg gggaaattgt tttaaaacac gcctatggta atacgagaac ataaaattct
     1801 gagaatgcgt attacacaac atattcgacg cgcaaaatat aactatatct cgtagcgaaa
     1861 actacagtaa tccttaaatg actactgtag cgattgtgtc gatttacggg ctcgattctc
     1921 ggaaaaaaaa atcatttttc aaattttgac agcgatattc aatttttctt cgttttttcg
     1981 tatttctcag tcatttttgt gcttattttt aatattttat tcattaataa attaatttaa
     2041 ttgaaaaacg tccagacgta tttttacatt tcccgtctat ttccgtaata ggcggttaat
     2101 aacgtagaaa tacgtctgta tgagtttact catcaaaatg acacgaatat tggacgtttt
     2161 taaatcaaat taatttatta ataggtaaaa tattaaaaaa taaacacaaa aatgacaaag
     2221 gaataggaaa acacgaagag aaattgagta tcgctgtcaa aattcgaaaa acgtggattt
     2281 ttccaagaat cgagcccgta aatcgacaca ttcgctacag tagtcattta aggattattg
     2341 tagttttcgc tacgagatac tttgcgcgcc aaaaacgtgg tgaaatacgc attctcagaa
     2401 ttttatgttc ccgtaatacc acaatgaccg aatatcataa taaaaaaatt caaaaacaat
     2461 ttctagattt tatatgattt tctgaaaatt gaaaaaatct cagttttcac ctaattatat
     2521 ttgaactaca gccaattgaa ctcgttcgtt ggagcgcgct tgcattattt tcattaatta
     2581 attttattaa tcctcattat ttcactgatt ttcttcattt ttgaggtttt ttttatcggg
     2641 aaatgaaaga aatatacaag aaaaatgcaa aatgtttatt aaaaactgaa attagtattt
     2701 ctcggagttt taggcatttt cagttacttt ttaataaaga ttttgcattt ttcttgtata
     2761 tttctttcat tttccgatta aaaaacccca aaaatgaaga aaatcagtga aataatgaaa
     2821 attaattcaa ataactaatg aaaataatgc aagcgcgctc caaccaacga gttcaattgg
     2881 cggtaattca aatataatta ggtgaaaact gagatttttt caattttcaa aaaatcatat
     2941 aacatctaga caattttttt gaattttttt atcatgatat tcggtcattg tgtccccata
     3001 ggcatgtttt aaagcaattt ccacctttaa ataaaatcga gaaaaaatgg cgtcaaaaga
     3061 catatgtaaa agctgtttct ctttgtatat tcacacagaa tatagcccga ttttggaggt
     3121 gaaaaaccgg tttttttgta ttctttacgc cacataaagt gatatggaag agaaaatcga
     3181 catatttatg tacttggagt acgagtacat tggagaaaaa gggtgaacta ctggaaaatc
     3241 tgggaaattt cagaaaaaaa ttccgaaaaa tcttttttgc tgaaaaaaaa atttgttcga
     3301 aatttgggaa atactggaaa attttcatca aaattaaagc gatttttaac gtttttgctt
     3361 gggagatact atttgtaatt ttaatgtttg gtgaggacaa cagaaatgac cttcagcgta
     3421 cggaaaacga ttaaaagaca catttgaatc gaaaatagcg tgaaaaacat gaaaatatcg
     3481 gaaaaatagc tttaaaattg gatttgaaag caaaaaatgt atcaaaaatc gagaaaaatg
     3541 cttgaaaaag ttgaaaaact caaacaaaaa atcaaaaaag gtttttttaa aaatctgtga
     3601 gtatcgcaac gaaagcacaa cttgaggcat gcgcctttaa accaaccgta accaaattcg
     3661 gaggcaaaaa aagatttctc gctgtttttt cagttttaaa atcacttttc acgtttcttt
     3721 tcgcagtaat tcgtttgcaa aacatctgaa aaaattttat tttataagaa attatataaa
     3781 aatcgctata aattgagttt ttgcccccaa attcagcacg gagcacttct caattaaata
     3841 taattttatt ttttaacatt gaaagctttc ataaaattga gctttgtttg taaataatca
     3901 gtgaaaaaac acagaaatct tcaaaaaata aagaaaaagt ttttaaaaat ctgtgagtcc
     3961 cgcaacgaaa ataaaacttt aggcatgcgc ctttaaacca accgtaacca aattcggcgg
     4021 caaaaatgga tttctcgccg ttttttcagt tttacaatca ctttttaggt ttctttttgc
     4081 agtatttcgt ttcccaaaca attaaaaatc aaattttctt ttccagatgg ctcaaacaaa
     4141 gccgattgcc gagcaaatgg gatcccccgg gctgcaggaa ttcgatatca agcttatcga
     4201 taccgtcgac ctcgaggggg cggtaccggt agaaaaaatg agtaaaggag aagaactttt
     4261 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     4321 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
     4381 cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc
     4441 tgattattta aattttcagc caacacttgt cactactttc tgttatggtg ttcaatgctt
     4501 ctcgagatac ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg
     4561 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtaagtt
     4621 taaacagttc ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt
     4681 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     4741 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     4801 agacaaacaa aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta
     4861 atctgattta aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca
     4921 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     4981 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     5041 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     5101 caaatgcccg ggggatcggt ggagctccac cggtggcggc cgctctagaa ctagtccacc
     5161 aaagccatct gccaagggag ccaagaaggc cgccaagacc gttacgaagc caaaggacgg
     5221 aaagaagaga cgtcatgccc gtaaggaatc atactccgtc tacatctacc gtgtcctcaa
     5281 gcaagttcat ccagacactg gagtttcctc caaagccatg tctatcatga actcttttgt
     5341 caacgatgtc ttcgagcgta ttgctgctga agcatcccgt cttgctcact acaacaagcg
     5401 ttccacaatc tcatcccgcg aaattcagac cgctgtccgt ctgatccttc caggagagct
     5461 tgccaagcac gccgtgtctg agggaaccaa ggccgttacc aagtacactt ccagcaagta
     5521 aactagtgga tcctaatttt gccgtatttt ccatattttg ttttgtatat ttatccactc
     5581 accccctctc tttgtcctgt gaatgaactt gtgccaaaaa agccaaaaat aatttacttt
     5641 ttttaaagac atattaaaac ttgaaaactt ggaacaatac ggtgcgaaag cgaaacagag
     5701 acagaaaaat gaacaaagag atccgtgaaa agtgaacgat gatttaggct tcttgttggg
     5761 cggcgtggat gagagtgatg ttgtctcctt tgagaagaat tctgccgatc ttgttgcgtc
     5821 ccttcgtctt catgttcacc tcctcggctt cgtcgaacac gacattcatg aactcgtcga
     5881 atccgatgat gtagccctcg agacggtggg tgacatcctc gtacaaccag atttggaccc
     5941 gagtgcggtt ttggagatat ctgaaatttg ggaaattgtt ttctactgat tagtgaaaaa
     6001 atattacctg aagatgaggt tcacaggctg aaccatcact ttgttgagct ttctcgtcga
     6061 catttttgcg ggatgatctg taaaaatcaa tatatttttt gaaagacaca aatttttaag
     6121 ataaatatca agaaaactcg attttagcag ctatttaagc tcaaaaacta caatttatgg
     6181 ctttaaaaca taatttttca cattttacat aaatttcaac gaaaaaaaat gccaaaaaac
     6241 ttcaaatttg agcagaaatc ggctattttc tcgacaaaaa taatttcgaa aattcgttca
     6301 aaaaagatga gtacgcaagc accgtgacgc acagttgcgg actttgtaaa ccgtacccct
     6361 gcataaaaag gcggaatttt gaatttaata aaatgcagtt tagaacaaag aaaagcacgg
     6421 agagaattgt gataactaat tatttgtatt taattttaaa tactacataa aactgaagat
     6481 tggaacgagt taaagctggt atttgcggtt tatagcccca aaagttaagg cttgttaggc
     6541 aattatcgat aaaaacaacg agaaattcgt gaaatcggca aaaatttata gaaaacgttt
     6601 gaattaaatt aaatttacgc ttttgccgcc aacacctatc gagaccctag ttataggggc
     6661 ggagcgtcat gggagcgcgc ttgcaactcg atttcaaaaa gtttcgctgt ttgtacaaca
     6721 ttctcagttg ttcttaatca gttaaataag tttcaaaatt ttcaaaatca taaaacacag
     6781 ttaatgctaa attaaagtct aaaaaatcag gtgataaggg gtttggacga ccaaaaagcg
     6841 accttcgctc gaaaattgat aaactaacta cactttttcc ggaaaaatga tggaaaaatc
     6901 ctgattttct tcgttgaatc aatttttgtt tcatttttaa cactttcaca tgaaatattt
     6961 cagttaaaat gctcaagcta attgggtttc ttataatttt gggtctcgca acggccgaca
     7021 agtgctcaga ttgtttggct caggggaaac agtgagttgg aagcttacaa ttctgagaat
     7081 tcgtattgcg cagcttattt gacgcgcaaa atatgaaata tcacgtagcg aaaactacag
     7141 taattcttta aataagacta ctgtagtgcc tatgtcgatt tactgcctca actttcttaa
     7201 tcaagctcgt aaatcgacac cagctctaca gtagtcatag ttttcgctac gagatatttt
     7261 gcgcgtcaaa tatggtgtgc aatacgcatt ttcagaattt tatgttctcg taatatacct
     7321 tttttcagct ggtgtgttac tgtaaatgca tgtggattta cgccatgtgt cactacaatt
     7381 acagtgagtt tttttgaaga aaaaaaaatt acccatttga tcgccaattc catttttcaa
     7441 aaatcaataa aaaaaaaaaa tttcggcgat cagaaacgtt acagaaagcc ctaaactgcc
     7501 cggaactacc agataaagcc cacgcttacg atgattcatt tatgaggaat aaggtattga
     7561 tcacacattc tgctgcctac aatatggacc cacaaaagtg ttttacgtag gttcaccttc
     7621 tagataaacc ttaaaaatcg atactttcag tagtaaaatg ccttcgatga aagtttccaa
     7681 aacagttaat gtcaattgtg atagatatgg gccaagtgag ttgttttatt agcccacgga
     7741 tttctggctt ccctcataaa ttgaaaagga agagattgcc gaactagacc attttggctc
     7801 ggcgcgttgc gtgtcacgtc gcggctcgat tgtaaaacta aatgtattgg tccgtgtgga
     7861 gtacacgact ttccctttcc ggcaggcgat tgtcaatgga gcgcgaaaaa ttcaatgagg
     7921 aaggccagaa ccccgtgtct gtagttttct caaaaagtgg caaaaactca ataattgcca
     7981 atttttgact gccaataaaa atttcactaa atttattaaa tttattttaa gttttattga
     8041 aattttttta aaatttcccc actggcgcta aaaaatccaa ttttcagaag caatgtgctt
     8101 cagcttcacg gccatagata ctgtccaaaa agtgattgca atgacgttta gagggacaga
     8161 aggagcagct caattgtctg aagaaatttt ggatttcttc cagtaagttc gggaaatggc
     8221 aatttaaagt ttaattggat ttttgtattt tttcttctca cgactacagt tttgcacatt
     8281 tttatgttat aaaaaaccaa aaaaaaattt ttttgccttt tttctcagct taccacaaaa
     8341 caatttcgac caaaaataaa ttgaaaaaat taaaaaattt tttgcacggt tttcggactc
     8401 aaaaaaagct gaatgttcct ggaactattt gtaaaattca aaattcaaaa ttttcacgaa
     8461 ttctctaaaa ggttccagca aattccacaa ctttcttgaa tttttcaaaa aagttcttga
     8521 gcattcaaca attatttttg gaattttcca aaggttacag gacgttccaa tttttcaaaa
     8581 aaatttctcg caaaaaatgt tttctcagaa aatttgaatt tccctccaca taaaagattg
     8641 ttctcaaaaa atttgcaaac acacattttc agcggaaaaa aggcgttctt cgactctgga
     8701 agtgtattcg aatatggtac ccagcttttg ttccctttag tgagggttaa ttccgagctt
     8761 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8821 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact
     8881 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8941 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9001 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9061 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9121 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9181 taggctcggc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9241 cccgacagga ctataaagat accaggcgtt cccccctgga agctccctcg tgcgctctcc
     9301 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9361 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9421 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9481 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9541 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9601 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9661 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9721 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9781 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9841 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9901 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9961 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactgcccg tcgtgtagat
    10021 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10081 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10141 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10201 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10261 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10321 agttacatga tcccccatgt tgtgaaaaaa agcggttagc tccttcggtc ctccgatcgt
    10381 tgtcagaagt aagttggccg cagtgttatc actcatgctt atggcagcac tgcataattc
    10441 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10501 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10561 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10621 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10681 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10741 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10801 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10861 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10921 acctg
//
