LOCUS       sequence-127593-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG306GPD-ccdB-ECFP
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..404
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(60..79)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
       61 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
      121 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      181 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
      241 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
      301 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
      361 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
      421 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
      481 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
      541 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
      601 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
      661 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
      721 tcatatatac tttagattga tttaaaactt
//
