LOCUS       sequence-127594-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TRC lentiviral vector for shRNA against mouse Dnd1 #3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLKO.1-shDnd1-No3
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(54..302)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(112..131)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(282..302)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(600..644)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aaagtgttcc tgaccaagtg tttactcgag taaacacttg gtcaggaaca cccggtgttt
       61 cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc
      121 atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac
      181 tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt aattccaatt
      241 atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc
      301 tcggtgaagg gggcggccgc tcgaggctag tctcgtgatc gataccgtcg agatccgttc
      361 actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc
      421 ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
      481 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
      541 actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac
      601 aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc
      661 gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc
      721 atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa
      781 ggcacagcag tggtgc
//
