LOCUS       sequence-127598-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TRC lentiviral vector for shRNA against mouse Dnd1 #4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLKO.1-shDnd1-No4
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..298)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(108..127)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(278..298)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(596..640)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agagcttact atgaatcttt ctcgagaaag attcatagta agctctgccg gtgtttcgtc
       61 ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat
      121 gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc
      181 tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt ccaattatct
      241 ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctcgg
      301 tgaagggggc ggccgctcga ggctagtctc gtgatcgata ccgtcgagat ccgttcacta
      361 atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt
      421 cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa
      481 ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta
      541 tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac
      601 ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt
      661 cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc
      721 aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca ttccaaggca
      781 cagcagtggt gcaaatgagt tttccagagc aacccc
//
