LOCUS       sequence-127607-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen lentiviral vector. Cre-removable and tet/dox-controllable 
            expression of FLAG-HA-tagged murine Dnd1 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLVE-FH-Dnd1-IN
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    57..174
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      200..209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             209..232
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             233..259
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtgtcgtgag gaatttcgac atttaaattt aattaatctc gacggtatcg gttaactttt
       61 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      121 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat
      181 cacgagacta gcctcgaggg ccaccatgga ctacaaggac gacgatgaca agtaccctta
      241 tgacgtgccc gattacgctt ctggtggcgg tggctcgggc ggaggtgggt cgggtggcgg
      301 cggatcagga tccatgcagt ccaaacggga gtgcgagcaa tggtgtgaga gggtgaatcc
      361 agagaacaag gcagccctgg aggcgtgggt aagggagact ggcatccgcc tggtgcaggt
      421 caacgggcaa aggaagtatg gagggccacc cccaggctgg gtgggcagcc cgccaccgtc
      481 agggtccgag gtgtatatcg gacgacttcc gcaggacgtg tacgagcacc agctaatccc
      541 actcttccag cgcgtggggc gcctctatga gttccgccta atgatgacct tcagtggttt
      601 gaaccgcggc tttgcctatg cgcgctacag ctcgcggcgc ggtgcccagg ccgccattgc
      661 tacgctgcac aacc
//
