LOCUS       sequence-12761-e       13063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13063)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 13063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2008 Nov 1. 323(1):76-87."
COMMENT     Sequence Label: pJH4.67
FEATURES             Location/Qualifiers
     source          1..13063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4206..4222)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4207..4223)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4276..4304)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5052..5072
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(10872..10890)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10911..10927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10911..10927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10924..10946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10935..10951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10959..10965,10966..10983,10984..10989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(11142..11159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11313..11901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(12072..12863,12864..12932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12695..12714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12933..13037)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctacgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaggggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctccaccgcg
      661 gtggcggccg cgcggaagat ctggaagtaa aatattgtaa tattttccct aaatttcact
      721 taaaaataag gatttttgca acgtaaaacc tcaaaaattc gctgaaattg acgatttttg
      781 ttcaaaattt tgaaaaacag ggttaatttt agctgaaaat cttcaatttt gccagaaatt
      841 ggcttttttt nccgtcgcca cacggagaac ttgccaaaaa tgaagacttt tagccaattt
      901 agccctgaaa attcgagttt tgggcgattt ttggtctgag aagccgtttt cgcagtatat
      961 tatattttct cgttaaattt cattttaaaa taaacatttt gaacgtataa ccccaaaaat
     1021 tcgctgcgca atggatgatt tttgtccaaa gttttgataa ttttagctaa aaacctcgaa
     1081 ttttggcaat ttttcgtgcc nngcatcggc cgaaagttga ctttttttgg aattatcata
     1141 atttaatgca cattttggta gtttatctca cataatctgt ctgcaggcgg gaaaaatttg
     1201 agataaacta ctaaaagatg caaaacaagg atgataattc cagaaaatca acttcggacc
     1261 gtctcgacac ggaaaattga gcgaaattgg agttttaaaa tgatttagcc ctgaaaattc
     1321 gagaaattta ggcggttttg ggtctgaaaa ggcgttttcg cagtattttc tncgctaaat
     1381 ttcatttatt atcacataat atcagtactt aggcgaatat cttgtagaaa tgttggtttt
     1441 gttcgataat tctctacttt tagagtcgat tcacgagaaa atgttgcacc atcttacttt
     1501 tttcccttgt aaaacataga ttcttctgta caatgaaaga gaacacaatt tcttcgtgaa
     1561 gcaactcaaa aagtagagaa aattcgaaca aaacccaaca tttctacaag atattcgtct
     1621 aaatagtgat ataatgtgtt aataagcata ttttaaatga aatttagcga aaaaactgcg
     1681 aaaatttgtc aaaaaagcga atttccatcc gatcagcaga tctctaaaag ttacataaaa
     1741 ttgaaagttt tgtggggaaa ttgttttaaa acacgcctat ggtaatacga gaacataaaa
     1801 ttctgagaat gcgtattaca caacatattc gacgcgcaaa atataactat atctcgtagc
     1861 gaaaactaca gtaatcctta aatgactact gtangcgatt gtgtcgattt acgggctcga
     1921 ttctcggaaa aaaaaatcat ttttcaaatt ttgacagcga tattcaattt ttcttcgttt
     1981 tttcgtatnt tctcagtcat ttttgtgctt atttttaata ttttattcat taataaattn
     2041 aatttaattg aaaaacgtcc agacgtattt ttacatttcc cgtctatttc cgtaataggc
     2101 ggttaataac gtagaaatac gtctgtatga gtttactcat caaaatgaca cgaatattgg
     2161 acgtttttaa atcaaattaa tttattaata ggtaaaatat taaaaaataa acacaaaaat
     2221 gacaaaggaa taggaaaaca cgaagagaaa ttgagtatcg ctgtncaaaa ttcgaaaaac
     2281 gtggattttt ccaagaatcg agcccgtaaa tcgacacatt cgctacagta gtcatttaag
     2341 gattattgta gttttcgcta cgagatactt tgcgcgccaa aaacgtggtg aaatacgcat
     2401 tctcagaatt ttatgttccc gtaataccac aatgaccgaa tatcataata aaaaaattca
     2461 aaaacaattt ctagatttta tatgattttc tgaaaattga aaaaatctca gttttcacct
     2521 aattatattt gaactacagc caattgaact cgttcgttgg agcgcgcttg cattattttc
     2581 attaattaat tttattaatc ctcattattt cactgatttt cttcattttt gaggtttttt
     2641 ttatcgggaa atgaaagaaa tatacaagaa aaatgcaaaa tgtttattaa aaactgaaat
     2701 tagtatttct cggagtttta ggcattttca gttacttttt aataaagatt ttgcattttt
     2761 cttgtatatt tctttcattt tccgattaaa aaaccccaaa aatgaagaaa atcagtgaaa
     2821 taatgaaaat taattcaaat aactaatgaa aataatgcaa gcgcgctcca accaacgagt
     2881 tcaattggcg gtaattcaaa tataattagg tgaaaactga gattttttca attttcaaaa
     2941 aatcatataa catctagaca atttttttga atttttttat catgatattc ggtcattgtg
     3001 tccccatagg catgttttaa agcaatttcc acctttaaat aaaatcgaga aaaaatggcg
     3061 tcaaaagaca tatgtaaaag ctgtttctct ttgtatattc acacagaata tagcccgatt
     3121 ttggaggtga aaaaccggtt tttttgtatt ctttacgcca cataaagtga tatggaagag
     3181 aaaatcgaca tatttatgta cttggagtac gagtacattg gagaaaaagg gtgaactact
     3241 ggaaaatctg ggaaatttca gaaaaaaatt ccgaaaaatc ttttttgctg aaaaaaaaat
     3301 ttgttcgaaa tttgggaaat actggaaaat tttcatcaaa attaaagcga tttttaacgt
     3361 ttttgcttgg gagatactat ttgtaatttt aatgtttggt gaggacaaca gaaatgacct
     3421 tcagcgtacg gaaaacgatt aaaagacaca tttgaatcga aaatagcgtg aaaaacatga
     3481 aaatatcgga aaaatagctt taaaattgga tttgaaagca aaaaatgtat caaaaatcga
     3541 gaaaaatgct tgaaaaagtt gaaaaactca aacaaaaaat caaaaaaggt ttttttaaaa
     3601 atctgtgagt atcgcaacga aagcacaact tgaggcatgc gcctttaaac caaccgtaac
     3661 caaattcgga ggcaaaaaaa gatttctcgc tgttttttca gttttaaaat cacttttcac
     3721 gtttcttttc gcagtaattc gtttgcaaaa catctgaaaa aattttattt tataagaaat
     3781 tatataaaaa tcgctataaa ttgagttttt gcccccaaat tcagcacgga gcacttctca
     3841 attaaatata attttatttt ttaacattga aagctttcat aaaattgagc tttgtttgta
     3901 aataatcagt gaaaaaacac agaaatcttc aaaaaataaa gaaaaagttt ttaaaaatct
     3961 gtgagtcccg caacgaaaat aaaactttag gcatgcgcct ttaaaccaac cgtaaccaaa
     4021 ttcggcggca aaaatggatt tctcgccgtt ttttcagttt tacaatcact ttttaggttt
     4081 ctttttgcag tatttcgttt cccaaacaat taaaaatcaa attttctttt ccagatggct
     4141 caaacaaagc cgattgccga gcaaatggga tcccccgggc tgcaggaatt cgatatcaag
     4201 cttatcgata ccgtcgacct cgagggggcg gtaccggtag aaaaaatgag taaaggagaa
     4261 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     4321 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     4381 tttatttgca ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta
     4441 actaaccctg attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt
     4501 caatgcttct cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
     4561 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     4621 cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa
     4681 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     4741 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     4801 atcatggcag acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta
     4861 actaactaat ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga
     4921 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     4981 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     5041 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     5101 gaactataca aatgcccggg ggatcggtgg agctccaccg gtggcggccg ctctagaact
     5161 agtatgtccg gtacgggtgc cttgatgact gtccacgttg gacagtgcgg aaatcagctt
     5221 gctcaagcct tctggaaatc gatggtcgac gaacatggaa tcaatgagta gggaataaat
     5281 ttgaggcacc gacaatttca attaggcaaa attcgatata ccacagtccg aaatgtgaaa
     5341 actattgcaa tttttatatt tcaatactga aatgctgcaa ttgtgaattg ttctcgtgaa
     5401 tcgacatgat tgaaaacaaa atatgaatta ttttactttt gtttgagatg aaacaaacat
     5461 ttttgctaag ttaaataatt taagacgcaa tttattcaag gtcataattc agcctcataa
     5521 ttttgataaa tataacaatt tttttcgttt tcagacgtgg acaaacaact cacgaagacg
     5581 atatgaatga taagaaagat ctattgttct accaggctga tgacgatcat tatgtgccaa
     5641 gagcagttct agtcgatcta gaacctcgtg taatcaacgg aatgatgcag tctccaaact
     5701 tttcgaatct tttcaatacg gacaatatat tcatgtccga tcacggaggt ggtgctggaa
     5761 ataattgggc aagtggatat tgtcaaggac agtgagttgt atttctttaa ttgaaaatca
     5821 atgataaata atatttaaga gaagttcaag agaagataat ggatatcatc attcgagagg
     5881 ctgaaaatac gaacaatttg gatggaattc tatttactca tagtgtttct ggaggaactg
     5941 gatctggaac gggatctctc ttgctcgaaa ggcttcgcga agcatttccg aagaaagtta
     6001 ttcaaactta cagtgttttt gcaaatagtg acaccagtac tgacgttgtt gttcatccat
     6061 ataactgggt actttctatg caaagactta ttgagaatcc tggtaagtta ataaattaaa
     6121 aacgaaatga caatgattta tcgaaaaatc tatgaaaatt ccgcaacaac gaacgtttga
     6181 aattacagta cccttaaaag gctcacatat attcgcatta agtgaaaaag accgggtacc
     6241 gtctcaattt ttgtatctga gtaataataa aaaaaaggaa tttcaaataa atagttttca
     6301 gatcatgttg ttgtcctcga taatgctgca ctgcaccgtc ttgcggctgg aaaattcaaa
     6361 acagatacac caacatttga tcatatcaat tctcttgtcg cccgaatcat gagcacttcc
     6421 actgctccat atcgattcaa ctctgcaatg tgcccatcaa ttcggtattt ggatttggct
     6481 ccgtttccac caatgcactt tattcaatcc ggtttgtttt tattccgaaa tatgttattt
     6541 tatctattta ttttgttttt tgcagctatc tctcctgttg tcgatccaaa tgagaacttt
     6601 acccgaaaaa cttcagtcgc tgacgtcact cgcttcttgt taaaaccaac ttcaatgatg
     6661 gtttcaactg cttctcgggt tcggccaaat gactgcatgc tatcagctta catgttcctt
     6721 caaggacaaa ttgaggcaca tacaattatg actgctgagc aaaacgttga tgtaagctca
     6781 atactttaaa gaatttccgg gaaaattgaa aaataaattt actaaatcaa ccataaaatt
     6841 caatttttat caaattttgt ttatgttcat gaaaactata agaaagcata taaatatttt
     6901 ctaaaaataa aaataagcaa ctttaaaaaa tatttttcga gatatcggaa aaacgaaaaa
     6961 ttttcgtaag ctaattttgg agatgtagat tttattctca aaacacgaag gcggtcacaa
     7021 ataccgagat actgcaaacg aaaaaaatct tcaaattaca ttgcaaaaat gagcgcttat
     7081 tttgacgaat cagcaattgt gaagcaaact tttttaaaaa gtatttttga catgaagtaa
     7141 aaaattttta gaaacttgga aatttgacaa aagcgtggca attcaaataa cacattagtc
     7201 agaaaatcaa aaatgcacca ataaaatcac tattttaaag acaaaattag tgattaataa
     7261 tgactttttt tcgttatttt tctataatcc accttaaagc atcaaatcaa taaaatcaaa
     7321 tttcagttcg caatcagaag gcctccattc tacatgctta aaccacttcg aatgatgcat
     7381 gctccactct ctccatatgt tcggccacaa tacaaagtat ctggactctt gctcaataac
     7441 agcacaagcg tggctccgtt attcgaatcg cttctctcca aatacgacaa actacgatca
     7501 aagagagcct tcatcgacaa gtttgaaaag attgacaatt tctcgctgga tatgatggac
     7561 gatgcaatgc acattgtgca agacttgctg gacgagtaca aggcggtcgt acaaaaggac
     7621 tatctgacaa gagggcttta gactagtgga tcctaatttt gccgtatttt ccatattttg
     7681 ttttgtatat ttatccactc accccctctc tttgtcctgt gaatgaactt gtgccaanaa
     7741 aagccanaaa ataatttact ttttttaaag acatattaaa acttgaaaac ttggaacaat
     7801 acggtgcgaa agcgaaacag agacagaaaa atgaacaaag agatccgtga aaagtgaacg
     7861 atgatttagg cttcttgttg ggcggcgtgg atgagagtga tgttgtctcc tttgagaaga
     7921 attctgccga tcttgttgcg tcccttcgtc ttcatgttca cctcctcggc ttcgtcgaac
     7981 acgacattca tgaactcgtc gaatccgatg atgtagccct cgagacggtg ggtgacatcc
     8041 tcgtacaacc agatttggac ccgagtgcgg ttttggagat atctgaaatt tgggaaattg
     8101 ttttctactg attagtgaaa aaatattacc tgaagatgag gttcacaggc tgaaccatca
     8161 ctttgttgag ctttctcgtc gacatttttg cgggatgatc tgtaaaaatc aatatatttn
     8221 tttgaaagac acaaattttt aagataaata tcaagaaaac tcgattttag cagctattta
     8281 agctcaaaaa ctacaattta tggctttaaa acataatttt tcacatttta cataaatttc
     8341 aacgaaaaaa aatgccaaaa aacttcaaat ttgagcagaa atcggctatt ttctcgacaa
     8401 aaataatttc gaaaattcgt tcaaaaaaga tgagtacgca agcaccgtga cgcacagttg
     8461 cggactttgt aaaccgtacc cctgcataaa aaggcggaat tttgaattta ataaaatgca
     8521 gtttagaaca aagaaaagca cggagagaat tgtgataact aattatttgt atttaatttt
     8581 aaatactaca taaaactgaa gattggaacg agttaaagct ggtatttgcg gtttatagcc
     8641 ccaaaagtta aggcttgtta ggcaattatc gataaaaaca acgagaaatt cgtgaaatcg
     8701 gcaaaaattt atagaaaacg tttgaattaa attaaattta cgcttttgcc gccaacacct
     8761 atcgagaccc tagttatagg ggcggagcgt cantgggagc gcgcttgcaa ctcgatttca
     8821 aaaagtttcg ctgtttgtac aacattctca gttgttctta atcagttaaa taagtttcaa
     8881 aattttnnca aaatcataaa acacagttaa tgctaaatta aagtctaaaa anatcaggtg
     8941 ataaggggtt tggacgacca aaaagcgacc ttcgctcgaa aattgataaa ctaactacac
     9001 tttttccgga aaaatgatgg aaaaatcctg attttcttcg ttgaatcaat ttttgtttca
     9061 tttttaacac tttcacatga nnaatatttc agttaaaatg ctcaagctaa ttgggtttct
     9121 tataattttg ggtctcgcaa cggccgacaa gtgctcagat tgtttggctc aggggaaaca
     9181 gtgagttgga agcttacaat tctgagaatt cgtattgcgc agcttatttg acgcgcaaaa
     9241 tatgaaatat cacgtagcga aaactacagt aattctttaa ataagactac tgtagtgcct
     9301 atgtcgattt actgcctcaa ctttcttaat caagctcgta aatcgacacc agctctacag
     9361 tagtcatagt tttcgctacg agatattttg cgcgtcaaat atggtgtgca atacgcattt
     9421 tcagaatttt atgttctcgt aatatacctt ttttcagctg gtgtgttact gtaaatgcat
     9481 gtggatttac gccatgtgtc actacaatta cagtgagttt tttntgaaga naaaaaaaat
     9541 tacccatttg atcgccaatt ccatttttca aaaatcaata aaaaaaaaaa atttcggcga
     9601 tcagaaacgt tacagaaagc cctaaactgc ccggaactac cagataaagc ccacgcttac
     9661 gatgattcat ttatgaggaa taaggtattg atcacacatt ctgctgccta caatatggac
     9721 ccacaaaagt gttttacgta ggttcacctt ctagataaac cttaaaaatc gatactttca
     9781 gtagtaaaat gccttcgatg aaagtttcca aaacagttaa tgtcaattgt gatagatatg
     9841 ggnccaagtg nagttgtttt attagcccac ggatttctgg cttccctcat aaattgaaaa
     9901 ggaagagatt gccgaactag accattttgg ctcggcgcgt tgcgtgtcac gtcgcggctc
     9961 gattgtaaaa ctaaatgtat tggtccgtgt ggagtacacg actttccctt tccggcaggc
    10021 gattgtcaat ggagcgcgaa aaattcaatg aggaaggcca gaaccccgtg tctgtagttt
    10081 tctcaaaaag tggcaaaaac tcaataattg ccaatttttg actgccaata aaaatttcac
    10141 taaatttatt aaatttattt taagttttat tgaaattntt tttaaaattt ccccactggc
    10201 gctaaaaaat ccaattttca gaagcaatgt gcttcagctt cacggccata gatactgtcc
    10261 aaaaagtgat tgcaatgacg tttagaggga cagaaggagc agctcaattg tctgaagaaa
    10321 ttttggattt cttccagtaa gttcgggaaa tggcaattta aagtttaatt ggatttttgt
    10381 attttttctt ctcacgacta cagttttgca catttttatg ttataaaaaa ccaaaaaaaa
    10441 atttttttgc cnttttttct cagcttacca caaaacaatt tcgaccaaaa ataaattgaa
    10501 aaaattaaaa aattttttgc acggttttcg gactcaaaaa aagctgaatg ttcctggaac
    10561 tatttgtaaa attcaaaatt caaaattttc acgaattctc taaaaggttc cagcaaattc
    10621 cacaactttc ttgaattttt caaaaaagtt cttgagcatt caacaattat ttttggaatt
    10681 ttccaaaggt tacaggacgt tccaattttt caaaaaaatt tctcgcaaaa aatnngtttt
    10741 ctcagaaaat ttgaatttcc ctccacataa aagattgttc tcaaaaaatt tgcaaacaca
    10801 cattttcagc nggaaaaaag gcgttcttcg actctggaag tgtattcgaa tatggtaccc
    10861 agcttttgtt ccctttagtg agggttaatt ccgagcttgg cgtaatcatg gtcatagctg
    10921 tttcctgtgt gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata
    10981 aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca
    11041 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    11101 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    11161 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    11221 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    11281 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctcggccc ccctgacgag
    11341 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    11401 caggcgttcc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    11461 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
    11521 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    11581 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    11641 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    11701 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    11761 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    11821 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    11881 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    11941 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12001 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    12061 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12121 cgttcatcca tagttgcctg actgcccgtc gtgtagataa ctacgatacg ggagggctta
    12181 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    12241 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    12301 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    12361 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    12421 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    12481 tgaaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    12541 gtgttatcac tcatgcttat ggcagcactg cataattctc ttactgtcat gccatccgta
    12601 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    12661 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    12721 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    12781 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    12841 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    12901 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    12961 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13021 caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
