LOCUS       sequence-127613-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen lentiviral vector. tet/dox controllable expression of 
            FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..36
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gatgacaagt acccttatga cgtgcccgat tacgcttctg gtggcggtgg ctcgggcgga
       61 ggtgggtcgg gtggcggcgg atcaggttcc atgcagtcca aacgggagtg cgagcaatgg
      121 tgtgagaggg tgaatccaga gaacaaggca gccctggagg cgtgggtaag ggagactggc
      181 atccgcctgg tgcaggtcaa cgggcaaagg aagtatggag ggccaccccc aggctgggtg
      241 ggcagcccgc caccgtcagg gtccgaggtg tatatcggac gacttccgca ggacgtgtac
      301 gagcaccagc taatcccact cttccagcgc gtggggcgcc tctatgagtt ccgcctaatg
      361 atgaccttca gtggtttgaa ccgcggcttt gccgcggcgc gctacagctc gcggcgcggt
      421 gcccaggccg ccattgctac gctgcacaac caccagctgc ggccttcttg ccagctcctg
      481 gtgtgccgaa gcaccgagaa gtgcgagctg acggtggatg gtctcccctt atcactcaac
      541 cgccgagcgt tactgctcgc cctgcaaccg ttcggtccct gcctccagga gacactgctg
      601 ctacccagcc ctgggtctgc gccctctcag attgcgctgc tcaagttcag tacgcaccga
      661 gctgctgcta tggccaaaaa ggccctggta gaaggtcagt cacgcctctg tggagagcag
      721 gtggctgtgg agtggctcaa gccagaccta aagcagcact tccgccagca gcttgcaggt
      781 ccctcactgc ggttcctcag gccagatgtc agccaactaa cccagaccag
//
