LOCUS       sequence-127616-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GAL-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..171)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(179..195)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(179..195)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(210..651)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(216..239)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    534..651
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(675..695)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(675..693)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(714..730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(714..730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(727..749)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    738..754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(762..768,769..786,787..792))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    807..828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(945..962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agccggaagc ataaagtgta aagcctgggg tgcctaatgc ggccgccata gtgactggat
       61 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      121 gatatttata tcattttacg tttctcgttc agcttttttg tacaaacttg tgatggggga
      181 tccactagtt ctagaatccg gggttttttc tccttgacgt taaagtatag aggtatatta
      241 acaatttttt gttgatactt ttattacatt tgaataagaa gtaatacaaa ccgaaaatgt
      301 tgaaagtatt agttaaagtg gttatgcagt ttttgcattt atatatctgt taatagatca
      361 aaaatcatcg cttcgctgat taattacccc agaaataagg ctaaaaaact aatcgcatta
      421 tcatcctatg gttgttaatt tgattcgttc atttgaaggt ttgtggggcc aggttactgc
      481 caatttttcc tcttcataac cataaaagct agtattgtag aatctttatt gttcggagca
      541 gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg gtgaagacga ggacgcacgg
      601 aggagagtct tccttcggag ggctgtcacc cgctcggcgg cttctaatcc gtactagagc
      661 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
      721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1081 gccaggaacc gtaaaaaggc cgcgttgc
//
