LOCUS       sequence-127620-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen lentiviral vector. tet/dox controllable expression of 
            FLAG-HA-tagged murine Dnd1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pCS-TRE-FHDnd1-Ubc-rtTA-I2G
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..17
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             18..44
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aggacgacga tgacaagtac ccttatgacg tgcccgatta cgcttctggt ggcggtggct
       61 cgggcggagg tgggtcgggt ggcggcggat caggttccat gcagtccaaa cgggagtgcg
      121 agcaatggtg tgagagggtg aatccagaga acaaggcagc cctggaggcg tgggtaaggg
      181 agactggcat ccgcctggtg caggtcaacg ggcaaaggaa gtatggaggg ccacccccag
      241 gctgggtggg cagcccgcca ccgtcagggt ccgaggtgta tatcggacga cttccgcagg
      301 acgtgtacga gcaccagcta atcccactct tccagcgcgt ggggcgcctc tatgagttcc
      361 gcctaatgat gaccttcagt ggtttgaacc gcggctttgc ctatgcgcgc tacagctcgc
      421 ggcgcggtgc ccaggccgcc attgctacgc tgcacaacca ccagctgcgg ccttcttgcc
      481 agctcctggt gtgccgaagc accgagaagt gcgagctgac ggtggatggt ctccccttat
      541 cactcaaccg ccgagcgtta ctgctcgccc tgcaaccgtt cggtccctgc ctccaggaga
      601 cactgctgct acccagccct gggtctgcgc cctctcagat tgcgctgctc aagttcagta
      661 cgcaccgagc tgctgctatg gccaaaaagg ccctggtaga aggtcagtca cgcctctgtg
      721 gagagcaggt ggctgtggag tggctcaagc cagacctaa
//
