LOCUS       sequence-127625-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein 
            with defective RRM (Y102A). Codons are optimized for bacteria 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pET11d-FHis-DND1-Y102A
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..58
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             83..100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catggactac aaagacgatg acgataaagc
       61 aagaagagca tctgtgcata tgcatcatca ccatcatcac ggctgccagt cgaaacgcga
      121 ttgcgaattg tggtgcgaac gtgttaaccc cgaaaataaa gcggccctcg aagcatgggt
      181 gcgcgaaacg ggcattcgtt tagtccaggt taacggtcag cgcaaatatg gtggtcctcc
      241 tccgggttgg gtagggtctc ctccgccggc gggcagcgaa gtgttcattg gccgcctccc
      301 gcaagatgtt tatgagcacc aactgattcc gctgttccaa cgtgtgggtc gtctctacga
      361 attccggctg atgatgacgt tttctggcct gaatcgtggg tttgctgctg cacgttattc
      421 tagccgtcgt ggtgcccagg cggctatcgc tacgctgcat aatcacccgc tgcgtccctc
      481 ctgtcctctc ttagtctgcc gtagcactga aaagtgtgaa ttgtctgtgg atggtcttcc
      541 gccaaacttg acccgttcgg cgctgttatt ggcactgcaa cccttaggcc cgggcctcca
      601 ggaagcacgc ctgcttccga gccccggccc ggctcctggg cagattgcac tcctcaaatt
      661 ttcatcacat cgtgcggcgg caatggccaa aaaagcattg
//
