LOCUS       sequence-127626-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein 
            with defective RRM (Y102A). Codons are optimized for bacteria 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pET11d-FHis-DND1-Y102A
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctttcgggc tttgttagca gccggatcct cactgtttta ccatcgtacc agcttcagcg
       61 cctgccgacc agagcaggtt cgccccactc tcactgaggg cctgcagcag ccgaaccgaa
      121 acggcatctt tggccacctc atggccatcg cggccgtcaa gcgtcagaac aacccaaatt
      181 aacccgctga acgggaccgg gtggcccgga atcacgactt gataccagaa acgatgccag
      241 cctgccggcc cgatgcccag gcatttcgtc agaaagacag gggagcccag cttcatacgc
      301 tgacagagaa gctgtaatgt tgcacgcgca ccctggaaac caagtttgtc ccgggccagt
      361 gcaagctggg aaccctccgg ctgcggtgaa cgcaagaacg gtcccaccaa ttgctgacgc
      421 aaccgctgtt tcagatcagg tttcagccac tccactgcga cctgttcacc acacaagtga
      481 gattggccct ccaccaatgc ttttttggcc attgccgccg cacgatgtga tgaaaatttg
      541 aggagtgcaa tctgcccagg agccgggccg gggctcggaa gcaggcgtgc ttcctggagg
      601 cccgggccta agggttgcag tgccaataac agcgccgaac gggtcaagtt tggcggaaga
      661 ccatccacag acaattcaca cttttcagtg ctacggcaga ctaagagagg acaggaggga
      721 cgcagcgggt gattatgcag cgtagcgata gccgcctggg caccacgacg gctagaataa
      781 cgtgcagcag caaacccacg attcaggcca g
//
