LOCUS       sequence-127628-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein. 
            Codons are optimized for bacteria expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pET11d-FHis-DND1
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             93..110
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catggactac aaagacgatg
       61 acgataaagc aagaagagca tctgtgcata tgcatcatca ccatcatcac ggctgccagt
      121 cgaaacgcga ttgcgaattg tggtgcgaac gtgttaaccc cgaaaataaa gcggccctcg
      181 aagcatgggt gcgcgaaacg ggcattcgtt tagtccaggt taacggtcag cgcaaatatg
      241 gtggtcctcc tccgggttgg gtagggtctc ctccgccggc gggcagcgaa gtgttcattg
      301 gccgcctccc gcaagatgtt tatgagcacc aactgattcc gctgttccaa cgtgtgggtc
      361 gtctctacga attccggctg atgatgacgt tttctggcct gaatcgtggg tttgcttatg
      421 cacgttattc tagccgtcgt ggtgcccagg cggctatcgc tacgctgcat aatcacccgc
      481 tgcgtccctc ctgtcctctc ttagtctgcc gtagcactga aaagtgtgaa ttgtctgtgg
      541 atggtcttcc gccaaacttg acccgttcgg cgctgttatt ggcactgcaa cccttaggcc
      601 cgggcctcca ggaagcacgc ctgcttccga gccccggccc ggctcctggg cagattgcac
      661 tcctcaaatt ttcatcacat cgtgcggcgg caatggccaa aaaagcattg gtggagggcc
      721 aatctcactt gtgtggtgaa caggtcgcag t
//
