LOCUS       sequence-12763-e       12142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2008 Nov 1. 323(1):76-87."
COMMENT     Sequence Label: pJH4.66
FEATURES             Location/Qualifiers
     source          1..12142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4206..4222)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4207..4223)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4276..4304)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5052..5072
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(9951..9969)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9990..10006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9990..10006)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10003..10025)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10014..10030
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10038..10044,10045..10062,10063..10068))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(10221..10238)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10392..10980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(11151..11942,11943..12011))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11774..11793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12012..12116)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctacgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaggggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctccaccgcg
      661 gtggcggccg cgcggaagat ctggaagtaa aatattgtaa tattttccct aaatttcact
      721 taaaaataag gatttttgca acgtaaaacc tcaaaaattc gctgaaattg acgatttttg
      781 ttcaaaattt tgaaaaacag ggttaatttt agctgaaaat cttcaatttt gccagaaatt
      841 ggcttttttt nccgtcgcca cacggagaac ttgccaaaaa tgaagacttt tagccaattt
      901 agccctgaaa attcgagttt tgggcgattt ttggtctgag aagccgtttt cgcagtatat
      961 tatattttct cgttaaattt cattttaaaa taaacatttt gaacgtataa ccccaaaaat
     1021 tcgctgcgca atggatgatt tttgtccaaa gttttgataa ttttagctaa aaacctcgaa
     1081 ttttggcaat ttttcgtgcc nngcatcggc cgaaagttga ctttttttgg aattatcata
     1141 atttaatgca cattttggta gtttatctca cataatctgt ctgcaggcgg gaaaaatttg
     1201 agataaacta ctaaaagatg caaaacaagg atgataattc cagaaaatca acttcggacc
     1261 gtctcgacac ggaaaattga gcgaaattgg agttttaaaa tgatttagcc ctgaaaattc
     1321 gagaaattta ggcggttttg ggtctgaaaa ggcgttttcg cagtattttc tncgctaaat
     1381 ttcatttatt atcacataat atcagtactt aggcgaatat cttgtagaaa tgttggtttt
     1441 gttcgataat tctctacttt tagagtcgat tcacgagaaa atgttgcacc atcttacttt
     1501 tttcccttgt aaaacataga ttcttctgta caatgaaaga gaacacaatt tcttcgtgaa
     1561 gcaactcaaa aagtagagaa aattcgaaca aaacccaaca tttctacaag atattcgtct
     1621 aaatagtgat ataatgtgtt aataagcata ttttaaatga aatttagcga aaaaactgcg
     1681 aaaatttgtc aaaaaagcga atttccatcc gatcagcaga tctctaaaag ttacataaaa
     1741 ttgaaagttt tgtggggaaa ttgttttaaa acacgcctat ggtaatacga gaacataaaa
     1801 ttctgagaat gcgtattaca caacatattc gacgcgcaaa atataactat atctcgtagc
     1861 gaaaactaca gtaatcctta aatgactact gtangcgatt gtgtcgattt acgggctcga
     1921 ttctcggaaa aaaaaatcat ttttcaaatt ttgacagcga tattcaattt ttcttcgttt
     1981 tttcgtatnt tctcagtcat ttttgtgctt atttttaata ttttattcat taataaattn
     2041 aatttaattg aaaaacgtcc agacgtattt ttacatttcc cgtctatttc cgtaataggc
     2101 ggttaataac gtagaaatac gtctgtatga gtttactcat caaaatgaca cgaatattgg
     2161 acgtttttaa atcaaattaa tttattaata ggtaaaatat taaaaaataa acacaaaaat
     2221 gacaaaggaa taggaaaaca cgaagagaaa ttgagtatcg ctgtncaaaa ttcgaaaaac
     2281 gtggattttt ccaagaatcg agcccgtaaa tcgacacatt cgctacagta gtcatttaag
     2341 gattattgta gttttcgcta cgagatactt tgcgcgccaa aaacgtggtg aaatacgcat
     2401 tctcagaatt ttatgttccc gtaataccac aatgaccgaa tatcataata aaaaaattca
     2461 aaaacaattt ctagatttta tatgattttc tgaaaattga aaaaatctca gttttcacct
     2521 aattatattt gaactacagc caattgaact cgttcgttgg agcgcgcttg cattattttc
     2581 attaattaat tttattaatc ctcattattt cactgatttt cttcattttt gaggtttttt
     2641 ttatcgggaa atgaaagaaa tatacaagaa aaatgcaaaa tgtttattaa aaactgaaat
     2701 tagtatttct cggagtttta ggcattttca gttacttttt aataaagatt ttgcattttt
     2761 cttgtatatt tctttcattt tccgattaaa aaaccccaaa aatgaagaaa atcagtgaaa
     2821 taatgaaaat taattcaaat aactaatgaa aataatgcaa gcgcgctcca accaacgagt
     2881 tcaattggcg gtaattcaaa tataattagg tgaaaactga gattttttca attttcaaaa
     2941 aatcatataa catctagaca atttttttga atttttttat catgatattc ggtcattgtg
     3001 tccccatagg catgttttaa agcaatttcc acctttaaat aaaatcgaga aaaaatggcg
     3061 tcaaaagaca tatgtaaaag ctgtttctct ttgtatattc acacagaata tagcccgatt
     3121 ttggaggtga aaaaccggtt tttttgtatt ctttacgcca cataaagtga tatggaagag
     3181 aaaatcgaca tatttatgta cttggagtac gagtacattg gagaaaaagg gtgaactact
     3241 ggaaaatctg ggaaatttca gaaaaaaatt ccgaaaaatc ttttttgctg aaaaaaaaat
     3301 ttgttcgaaa tttgggaaat actggaaaat tttcatcaaa attaaagcga tttttaacgt
     3361 ttttgcttgg gagatactat ttgtaatttt aatgtttggt gaggacaaca gaaatgacct
     3421 tcagcgtacg gaaaacgatt aaaagacaca tttgaatcga aaatagcgtg aaaaacatga
     3481 aaatatcgga aaaatagctt taaaattgga tttgaaagca aaaaatgtat caaaaatcga
     3541 gaaaaatgct tgaaaaagtt gaaaaactca aacaaaaaat caaaaaaggt ttttttaaaa
     3601 atctgtgagt atcgcaacga aagcacaact tgaggcatgc gcctttaaac caaccgtaac
     3661 caaattcgga ggcaaaaaaa gatttctcgc tgttttttca gttttaaaat cacttttcac
     3721 gtttcttttc gcagtaattc gtttgcaaaa catctgaaaa aattttattt tataagaaat
     3781 tatataaaaa tcgctataaa ttgagttttt gcccccaaat tcagcacgga gcacttctca
     3841 attaaatata attttatttt ttaacattga aagctttcat aaaattgagc tttgtttgta
     3901 aataatcagt gaaaaaacac agaaatcttc aaaaaataaa gaaaaagttt ttaaaaatct
     3961 gtgagtcccg caacgaaaat aaaactttag gcatgcgcct ttaaaccaac cgtaaccaaa
     4021 ttcggcggca aaaatggatt tctcgccgtt ttttcagttt tacaatcact ttttaggttt
     4081 ctttttgcag tatttcgttt cccaaacaat taaaaatcaa attttctttt ccagatggct
     4141 caaacaaagc cgattgccga gcaaatggga tcccccgggc tgcaggaatt cgatatcaag
     4201 cttatcgata ccgtcgacct cgagggggcg gtaccggtag aaaaaatgag taaaggagaa
     4261 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     4321 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     4381 tttatttgca ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta
     4441 actaaccctg attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt
     4501 caatgcttct cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
     4561 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     4621 cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa
     4681 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     4741 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     4801 atcatggcag acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta
     4861 actaactaat ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga
     4921 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     4981 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     5041 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     5101 gaactataca aatgcccggg ggatcggtgg agctccaccg gtggcggccg ctctagaact
     5161 agtatgagag agatcgtcca cgtgcaagcc ggacaatgcg gaaaccaaat cggatccaaa
     5221 ttctgggagg tcatctccga cgagcacgga atccagccag acggaacctt caagggagag
     5281 acggatctcc agcttgagcg cattgacgtc tactacaacg aagccaacag tgagttttta
     5341 aaattttctg gagatttcga aatcgtttag ttggaatgat taattaatta aattttttca
     5401 gacggaaagt atgtgccacg cgccgtgttg gtcgatctcg aaccaggaac catggattct
     5461 gtccgctctg gaccattcgg ccagctgttc cgtcctgaca actttgtgtt cggacaaagc
     5521 ggagccggaa acaactgggc caagggtcac tacacagaag gagctgagct tgtcgacaat
     5581 gtgcttgacg tgatccgcaa ggaagccgaa ggatgcgatt gtcttcaagg attccaactc
     5641 actcactctc tcggaggagg aaccggatct ggaatgggaa cacttctcat ctccaaaatc
     5701 cgcgaagagt acccagacag aatcatgagc tcattctcgg ttgtaccatc gccaaaggtg
     5761 tccgacacag ttgtagaacc atacaatgcc accctctccg tccaccagct tgttgagaac
     5821 accgatgaga cctactgcat tgacaacgag gccctctacg acatctgcta ccgtaccctc
     5881 aaactcacca acccaaccta cggtgatttg aaccatcttg gtaagttatt atttcttgtt
     5941 caagtttaag ttatgcacaa gttacccaaa ttgaaaccta ataattacaa ttttcagtat
     6001 ccctcaccat gtccggagtt accacctgtc tccgattccc aggacagctc aatgccgatc
     6061 tccgcaagct tgccgtcaac atggttccat tcccacgtct tcacttcttc atgccaggat
     6121 tcgcaccact ttccgccaag ggaacccagg cttaccgtgc ccttaccgtc gccgagctca
     6181 cccaacaaat gttcgatgcc aagaacatga tggctgcctg tgacccaaga cacggacgtt
     6241 acctgaccgt ggccgctatg ttccgtggac gcatgtcgat gagagaggtt gacgagcaaa
     6301 tgctcaacgt tcagaacaag aactcgtcct acttcgtcga atggatcccg aacaacgtca
     6361 agaccgccgt ttgtgacatc ccaccaagag gactcaagat ggctgccacc ttcgttggaa
     6421 actcgaccgc tatccaagag cttttcaagc gtatctcaga gcagttcact ggtaagaaat
     6481 ttgaaactat catccagtat cagttttaaa aacgttattt tttcagctat gttccgccgc
     6541 aaggctttcc tccattggta cactggagaa ggaatggacg agatggagtt caccgaagcc
     6601 gagagcaaca tgaacgatct catctcagaa taccaacaat accaagaagc aactgccgaa
     6661 gacgacgtcg acggatacgc tgagggagaa gctggagaga cttacgaatc ggagcaataa
     6721 actagtggat cctaattttg ccgtattttc catattttgt tttgtatatt tatccactca
     6781 ccccctctct ttgtcctgtg aatgaacttg tgccaanaaa agccanaaaa taatttactt
     6841 tttttaaaga catattaaaa cttgaaaact tggaacaata cggtgcgaaa gcgaaacaga
     6901 gacagaaaaa tgaacaaaga gatccgtgaa aagtgaacga tgatttaggc ttcttgttgg
     6961 gcggcgtgga tgagagtgat gttgtctcct ttgagaagaa ttctgccgat cttgttgcgt
     7021 cccttcgtct tcatgttcac ctcctcggct tcgtcgaaca cgacattcat gaactcgtcg
     7081 aatccgatga tgtagccctc gagacggtgg gtgacatcct cgtacaacca gatttggacc
     7141 cgagtgcggt tttggagata tctgaaattt gggaaattgt tttctactga ttagtgaaaa
     7201 aatattacct gaagatgagg ttcacaggct gaaccatcac tttgttgagc tttctcgtcg
     7261 acatttttgc gggatgatct gtaaaaatca atatatttnt ttgaaagaca caaattttta
     7321 agataaatat caagaaaact cgattttagc agctatttaa gctcaaaaac tacaatttat
     7381 ggctttaaaa cataattttt cacattttac ataaatttca acgaaaaaaa atgccaaaaa
     7441 acttcaaatt tgagcagaaa tcggctattt tctcgacaaa aataatttcg aaaattcgtt
     7501 caaaaaagat gagtacgcaa gcaccgtgac gcacagttgc ggactttgta aaccgtaccc
     7561 ctgcataaaa aggcggaatt ttgaatttaa taaaatgcag tttagaacaa agaaaagcac
     7621 ggagagaatt gtgataacta attatttgta tttaatttta aatactacat aaaactgaag
     7681 attggaacga gttaaagctg gtatttgcgg tttatagccc caaaagttaa ggcttgttag
     7741 gcaattatcg ataaaaacaa cgagaaattc gtgaaatcgg caaaaattta tagaaaacgt
     7801 ttgaattaaa ttaaatttac gcttttgccg ccaacaccta tcgagaccct agttataggg
     7861 gcggagcgtc antgggagcg cgcttgcaac tcgatttcaa aaagtttcgc tgtttgtaca
     7921 acattctcag ttgttcttaa tcagttaaat aagtttcaaa attttnncaa aatcataaaa
     7981 cacagttaat gctaaattaa agtctaaaaa natcaggtga taaggggttt ggacgaccaa
     8041 aaagcgacct tcgctcgaaa attgataaac taactacact ttttccggaa aaatgatgga
     8101 aaaatcctga ttttcttcgt tgaatcaatt tttgtttcat ttttaacact ttcacatgan
     8161 naatatttca gttaaaatgc tcaagctaat tgggtttctt ataattttgg gtctcgcaac
     8221 ggccgacaag tgctcagatt gtttggctca ggggaaacag tgagttggaa gcttacaatt
     8281 ctgagaattc gtattgcgca gcttatttga cgcgcaaaat atgaaatatc acgtagcgaa
     8341 aactacagta attctttaaa taagactact gtagtgccta tgtcgattta ctgcctcaac
     8401 tttcttaatc aagctcgtaa atcgacacca gctctacagt agtcatagtt ttcgctacga
     8461 gatattttgc gcgtcaaata tggtgtgcaa tacgcatttt cagaatttta tgttctcgta
     8521 atataccttt tttcagctgg tgtgttactg taaatgcatg tggatttacg ccatgtgtca
     8581 ctacaattac agtgagtttt ttntgaagan aaaaaaaatt acccatttga tcgccaattc
     8641 catttttcaa aaatcaataa aaaaaaaaaa tttcggcgat cagaaacgtt acagaaagcc
     8701 ctaaactgcc cggaactacc agataaagcc cacgcttacg atgattcatt tatgaggaat
     8761 aaggtattga tcacacattc tgctgcctac aatatggacc cacaaaagtg ttttacgtag
     8821 gttcaccttc tagataaacc ttaaaaatcg atactttcag tagtaaaatg ccttcgatga
     8881 aagtttccaa aacagttaat gtcaattgtg atagatatgg gnccaagtgn agttgtttta
     8941 ttagcccacg gatttctggc ttccctcata aattgaaaag gaagagattg ccgaactaga
     9001 ccattttggc tcggcgcgtt gcgtgtcacg tcgcggctcg attgtaaaac taaatgtatt
     9061 ggtccgtgtg gagtacacga ctttcccttt ccggcaggcg attgtcaatg gagcgcgaaa
     9121 aattcaatga ggaaggccag aaccccgtgt ctgtagtttt ctcaaaaagt ggcaaaaact
     9181 caataattgc caatttttga ctgccaataa aaatttcact aaatttatta aatttatttt
     9241 aagttttatt gaaattnttt ttaaaatttc cccactggcg ctaaaaaatc caattttcag
     9301 aagcaatgtg cttcagcttc acggccatag atactgtcca aaaagtgatt gcaatgacgt
     9361 ttagagggac agaaggagca gctcaattgt ctgaagaaat tttggatttc ttccagtaag
     9421 ttcgggaaat ggcaatttaa agtttaattg gatttttgta ttttttcttc tcacgactac
     9481 agttttgcac atttttatgt tataaaaaac caaaaaaaaa tttttttgcc nttttttctc
     9541 agcttaccac aaaacaattt cgaccaaaaa taaattgaaa aaattaaaaa attttttgca
     9601 cggttttcgg actcaaaaaa agctgaatgt tcctggaact atttgtaaaa ttcaaaattc
     9661 aaaattttca cgaattctct aaaaggttcc agcaaattcc acaactttct tgaatttttc
     9721 aaaaaagttc ttgagcattc aacaattatt tttggaattt tccaaaggtt acaggacgtt
     9781 ccaatttttc aaaaaaattt ctcgcaaaaa atnngttttc tcagaaaatt tgaatttccc
     9841 tccacataaa agattgttct caaaaaattt gcaaacacac attttcagcn ggaaaaaagg
     9901 cgttcttcga ctctggaagt gtattcgaat atggtaccca gcttttgttc cctttagtga
     9961 gggttaattc cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    10021 ccgctcacaa ttccacacaa cataggagcc ggaagcataa agtgtaaagc ctggggtgcc
    10081 taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    10141 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    10201 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    10261 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    10321 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    10381 ttgctggcgt ttttccatag gctcggcccc cctgacgagc atcacaaaaa tcgacgctca
    10441 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgttccc ccctggaagc
    10501 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    10561 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
    10621 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    10681 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    10741 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    10801 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    10861 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    10921 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    10981 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    11041 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    11101 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    11161 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    11221 ctgcccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    11281 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    11341 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    11401 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    11461 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    11521 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gaaaaaaagc ggttagctcc
    11581 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catgcttatg
    11641 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    11701 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    11761 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    11821 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    11881 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    11941 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    12001 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    12061 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    12121 tttccccgaa aagtgccacc tg
//
