LOCUS       sequence-127631-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a 
            level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pPAt2S3 (GB0029)
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(505..523)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(506..523)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(541..557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(541..557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(554..576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    565..581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(589..595,596..613,614..619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    634..655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgcattgttt
       61 tgctatttgt gtatgttttc ttgttttggt ttgagtgaag agtgtagtga agggttggtt
      121 tgtatttata ggtgagaaag gagatttgca tggcaatcac gtgtaagaat gcatgcatgc
      181 atgagtgtgt gtggagagag agagatgctc ttacgttgtt gtcgggatta tgtgttggaa
      241 aagagtgaca agtgacgaac aaaacaatac cactcatcat agctccgctt atgcatgcta
      301 tgtgtgattt gtagctcttg ttctgacatg acactatgaa caatttttta aaaagaaggt
      361 taaactttta tttaaataaa aaccctatgt taatttggtt tcctccngag accactcatc
      421 gcccaatcac tagtgaattc gcggccgcct gcaggtcgac catatgggag agctcccaac
      481 gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg
      541 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      601 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      661 gttgcgctca ctgcccgctt tccagtc
//
