LOCUS       sequence-127637-        6391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes Cep123 (CCDC123/Cep89) isoform 1 with an N-ter 
            Myc-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Sillibourne JE, Hurbain I, Grand-Perret T, Goud B, Tran P, Bornens M
  TITLE     Primary ciliogenesis requires the distal appendage component Cep123.
  JOURNAL   Biol Open. 2013 Apr 9;2(6):535-45. doi: 10.1242/bio.20134457. Print 
            2013 Jun 15.
  PUBMED    23789104
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/bio.20134457"; journalName: "Biol Open"; date: "2013-04-9- 
            9"; volume: "2"; issue: "6"; pages: "535-45"
COMMENT     Sequence Label: pMyc-C1-Cep123 (CCDC123/Cep89)
FEATURES             Location/Qualifiers
     source          1..6391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..33
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    2569..2690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2606..2625)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2660..2679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2697..3152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2834..2855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3046..3065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3179..3283
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3285..3642
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3285..3476
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3308..3328)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3493..3628
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3677..4471
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3677..4471
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3731..3750)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4341..4360
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4659..4678)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4703..4750
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5079..5667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5568..5587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5842..6145
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6146..6349
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6300..6320
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggaacaaa aacttatttc tgaagaagat ctgctgtaca agtccggact cagatctcga
       61 gctcaagctt cgaattctgc agtcgacatg ctcctgggat ttcggagagg ccgcaggagt
      121 catttcaaac acatcatcca tggcctttta cctgcagcca gcgttgctcc gaaggcagct
      181 gtgccacgca cacctcctcc ccgcagcccc aacccatctc cagagagacc aagatctgct
      241 ctggcagcag ccattctggc gacaacattg actgggcgga cggttgctat tcctcagcct
      301 cgccagaggt cccggtctga gagtgatgtg agcagtgttg aacaggacag cttcatcgag
      361 ccctatgcca ccacctcaca gctgaggcct cgaccaaatt ggcagagtga gatgggaaga
      421 agatcttctt tgccatcctt tgaaacactg gactatgggg acgaagagga cattgaaact
      481 cagctgtcat ccagcggcaa ggaattgggg gatgtcagtg cccgggagga cagaggaggc
      541 cacagtgatg acctgtacgc tgtgccacac agaaatcagg tgccattgtt acatgaggtg
      601 aacagtgaag acgatgaaaa tatttctcat caagatgggt ttccaggctc ccctcctgca
      661 ccacagcgga cacaacaaaa agatggtaaa caccctgttc tgaatttaaa ggatgaaaaa
      721 cctccattat gtgagaaacc tccaccctcc ccagatataa ctggtagagc acgtcaaaga
      781 tatacagaaa taaccagaga aaagtttgag gcattaaaag aagaaaatat ggacctaaac
      841 aatatgaatc aaagccttac ccttgaacta aacacaatga aacaagcaat gaaagaacta
      901 cagttaaaac ttaagggaat ggaaaaagag aagagaaagc tcaaagaggc tgagaaggcg
      961 tcgtcacagg aagttgctgc acctgaatta ctttatctgc gaaaacaagc tcaagaactg
     1021 gtggatgaaa atgatggatt gaaaatgact gtccatcgtt tgaatgtaga actcagtcga
     1081 tatcagacaa aattcaggca tttgtccaag gaagagagct taaatattga aggcctccca
     1141 tccaagggcc ctataccacc ctggttgttg gatataaagt acctgtcacc attgttgctg
     1201 gcttatgaag atatgatgaa agagaaggac gagctcaatg ccaccctcaa ggaggaaatg
     1261 agaatgttta ggatgcgagc ccaagaagtg gtgaaagaaa atgaagaatt gcaccaagag
     1321 ttaaataaga gtagtgctgt taccagtgag gaatggcgtc agcttcagac tcaagcaaaa
     1381 ctggttttag aggaaaacaa gttgttgctg gagcagttgg agattcagca aaggaaagcc
     1441 aaggacagcc accaggagcg cctccaagaa gtttctaagc tgactaaaca actaatgctc
     1501 ctggaggcaa aaacccacgg ccaggaaaag gagctggcgg agaacaggga acagctggag
     1561 attttacgtg ccaaatgcca agaactcaaa acacactcgg atggcaaaat cgcagtggaa
     1621 gttcataaat caattgtgaa tgaattaaaa agccaattac agaaggaaga agagaaagaa
     1681 agggctgaga tggaggagtt gatggagaag ctgacagtcc tgcaagcgca gaagaagagc
     1741 ctgctgttag agaagaacag tttgacagag caaaacaaag cactggaagc cgaacttgaa
     1801 cgagcacaga aaatcaatag gaaatctcaa aagaaaattg aggtcctcaa aaagcaggtg
     1861 gaaaaagcca tggggaacga aatgtctgct catcagtacc tggcaaacct tgttggcctg
     1921 gcagaaaata taacccagga acgtgacagt cttatgtgtt tggcaaaatg tttagaaagt
     1981 gagaaggatg gagtgcttaa taaagtcata aaaagcaaca ttcgcctggg aaagttagag
     2041 gaaaaagtca agggctacaa gaagcaggca gcactgaagc tgggggacat cagtcaccgt
     2101 ctgctggagc agcaggagga cttcgccggc aagacagccc agtaccggca ggagatgcgg
     2161 cacctgcacc aggtgctgaa ggacaagcag gaggtgctgg accaggcgct gcagcagaac
     2221 agagaaatgg aaggtgaact tgaagttatt tgggaatcta ccttcaggga aaaccgaaga
     2281 atccgagaac ttctccagga cacactcacg aggacaggcg tgcaggacaa ccccagagct
     2341 ctggttgccc ccagcctcaa tggcgtctct caggcagacc tgctggacgg ctgcgatgtc
     2401 tgctcctatg acctgaagtc tcatgccccc acctgctagg gatccaccgg atctagataa
     2461 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2521 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2581 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2641 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     2701 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3121 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3181 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3241 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3301 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3361 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3421 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3481 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3541 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3601 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     3661 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3721 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3781 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3841 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3901 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3961 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4021 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4081 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4141 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4201 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4261 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4321 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4381 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4441 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4501 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4561 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4621 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     4681 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     4741 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4801 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4861 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     4921 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4981 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5221 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5701 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5761 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
     5821 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     5881 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     5941 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     6001 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     6061 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     6121 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     6181 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     6241 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     6301 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     6361 ccgtcagatc cgctagcgct accggtccac c
//
