LOCUS       sequence-127638-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and 
            C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet 
            P, Ramaekers FC, Bornens M, Grand-Perret T
  TITLE     Autophosphorylation of polo-like kinase 4 and its role in centriole 
            duplication.
  JOURNAL   Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 
            10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
  PUBMED    20032307
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-06-0505"; journalName: "Mol Biol Cell"; date: 
            "2010-02-15- 15"; volume: "21"; issue: "4"; pages: "547-61"
COMMENT     Sequence Label: pEGFP-C3-PLK4-3xFLAG
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     153..174
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
       61 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
      121 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      181 gccgggatca ctctcggcat ggacgagctg tacaagtact cagatctcga gctcaagctt
      241 ggtaccatgg cgacctgcat cggggagaag atcgaggatt ttaaagttgg aaatctgctt
      301 ggtaaaggat catttgctgg tgtctacaga gctgagtcca ttcacactgg tttggaagtt
      361 gcaatcaaaa tgatagataa gaaagccatg tacaaagcag gaatggtaca gagagtccaa
      421 aatgaggtga aaatacattg ccaattgaaa catccttcta tcttggagct ttataactat
      481 tttgaagata gcaattatgt gtatctggta ttagaaatgt gccataatgg agaaatgaac
      541 aggtatctaa agaatagagt gaaacccttc tcagaaaatg aagctcgaca cttcatgcac
      601 cagatcatca cagggatgtt gtatcttcat tctcatggta tactacaccg ggacctcaca
      661 ctttctaacc tcctactgac tcgtaatatg aacatcaaga ttgctgattt tgggctggca
      721 actcaactga aaatgccaca tgaaaagcac tatacattat gtggaactcc taactacatt
      781 tcaccagaaa ttgccactcg aagtgcacat ggcctt
//
