LOCUS       sequence-12764-e       12296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12296)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 12296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2008 Nov 1. 323(1):76-87."
COMMENT     Sequence Label: pKR2.40
FEATURES             Location/Qualifiers
     source          1..12296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4206..4222)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4207..4223)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4276..4304)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5052..5072
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    5167..5291
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5316..5321,5322..5339,5340..5346)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5400..6059
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5466..5485)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6401..6706
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6585..6604
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6747..6871)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(10105..10123)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10144..10160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10144..10160)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10157..10179)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10168..10184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10192..10198,10199..10216,10217..10222))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(10375..10392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10546..11134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(11305..12096,12097..12165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11928..11947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12166..12270)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctacgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaggggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctccaccgcg
      661 gtggcggccg cgcggaagat ctgctgatcg gatggaaatt cgcttttttg acaaattttc
      721 gcagtttttt cgctaaattt catttaaaat atgcttatta acacattata tcactattta
      781 gacgaatatc ttgtagaaat gttgggtttt gttcgaattt tctctacttt ttgagttgct
      841 tcacgaagaa attgtgttct ctttcattgt acagaagaat ctatgtttta caagggaaaa
      901 aagtaagatg gtgcaacatt ttctcgtgaa tcgactctaa aagtagagaa ttatcgaaca
      961 aaaccaacat ttctacaaga tattcgccta agtactgata ttatgtgata ataaatgaaa
     1021 tttagcgnag aaaatactgc gaaaacgcct tttcagaccc aaaaccgcct aaatttctcg
     1081 aattttcagg gctaaatcat tttaaaactc caatttcgct caattttccg tgtcgagacg
     1141 gtccgaagtt gattttctgg aattatcatc cttgttttgc atcttttagt agtttatctc
     1201 aaatttttcc cgcctgcaga cagattatgt gagataaact accaaaatgt gcattaaatt
     1261 atgataattc caaaaaaagt caactttcgg ccgatgcnng gcacgaaaaa ttgccaaaat
     1321 tcgaggtttt tagctaaaat tatcaaaact ttggacaaaa atcatccatt gcgcagcgaa
     1381 tttttggggt tatacgttca aaatgtttat tttaaaatga aatttaacga gaaaatataa
     1441 tatactgcga aaacggcttc tcagaccaaa aatcgcccaa aactcgaatt ttcagggcta
     1501 aattggctaa aagtcttcat ttttggcaag ttctccgtgt ggcgacggna aaaaaagcca
     1561 atttctggca aaattgaaga ttttcagcta aaattaaccc tgtttttcaa aattttgaac
     1621 aaaaatcgtc aatttcagcg aatttttgag gttttacgtt gcaaaaatcc ttatttttaa
     1681 gtgaaattta gggaaaatat tacaatattt tacttccaga tctctaaaag ttacataaaa
     1741 ttgaaagttt tgtggggaaa ttgttttaaa acacgcctat ggtaatacga gaacataaaa
     1801 ttctgagaat gcgtattaca caacatattc gacgcgcaaa atataactat atctcgtagc
     1861 gaaaactaca gtaatcctta aatgactact gtangcgatt gtgtcgattt acgggctcga
     1921 ttctcggaaa aaaaaatcat ttttcaaatt ttgacagcga tattcaattt ttcttcgttt
     1981 tttcgtatnt tctcagtcat ttttgtgctt atttttaata ttttattcat taataaattn
     2041 aatttaattg aaaaacgtcc agacgtattt ttacatttcc cgtctatttc cgtaataggc
     2101 ggttaataac gtagaaatac gtctgtatga gtttactcat caaaatgaca cgaatattgg
     2161 acgtttttaa atcaaattaa tttattaata ggtaaaatat taaaaaataa acacaaaaat
     2221 gacaaaggaa taggaaaaca cgaagagaaa ttgagtatcg ctgtncaaaa ttcgaaaaac
     2281 gtggattttt ccaagaatcg agcccgtaaa tcgacacatt cgctacagta gtcatttaag
     2341 gattattgta gttttcgcta cgagatactt tgcgcgccaa aaacgtggtg aaatacgcat
     2401 tctcagaatt ttatgttccc gtaataccac aatgaccgaa tatcataata aaaaaattca
     2461 aaaacaattt ctagatttta tatgattttc tgaaaattga aaaaatctca gttttcacct
     2521 aattatattt gaactacagc caattgaact cgttcgttgg agcgcgcttg cattattttc
     2581 attaattaat tttattaatc ctcattattt cactgatttt cttcattttt gaggtttttt
     2641 ttatcgggaa atgaaagaaa tatacaagaa aaatgcaaaa tgtttattaa aaactgaaat
     2701 tagtatttct cggagtttta ggcattttca gttacttttt aataaagatt ttgcattttt
     2761 cttgtatatt tctttcattt tccgattaaa aaaccccaaa aatgaagaaa atcagtgaaa
     2821 taatgaaaat taattcaaat aactaatgaa aataatgcaa gcgcgctcca accaacgagt
     2881 tcaattggcg gtaattcaaa tataattagg tgaaaactga gattttttca attttcaaaa
     2941 aatcatataa catctagaca atttttttga atttttttat catgatattc ggtcattgtg
     3001 tccccatagg catgttttaa agcaatttcc acctttaaat aaaatcgaga aaaaatggcg
     3061 tcaaaagaca tatgtaaaag ctgtttctct ttgtatattc acacagaata tagcccgatt
     3121 ttggaggtga aaaaccggtt tttttgtatt ctttacgcca cataaagtga tatggaagag
     3181 aaaatcgaca tatttatgta cttggagtac gagtacattg gagaaaaagg gtgaactact
     3241 ggaaaatctg ggaaatttca gaaaaaaatt ccgaaaaatc ttttttgctg aaaaaaaaat
     3301 ttgttcgaaa tttgggaaat actggaaaat tttcatcaaa attaaagcga tttttaacgt
     3361 ttttgcttgg gagatactat ttgtaatttt aatgtttggt gaggacaaca gaaatgacct
     3421 tcagcgtacg gaaaacgatt aaaagacaca tttgaatcga aaatagcgtg aaaaacatga
     3481 aaatatcgga aaaatagctt taaaattgga tttgaaagca aaaaatgtat caaaaatcga
     3541 gaaaaatgct tgaaaaagtt gaaaaactca aacaaaaaat caaaaaaggt ttttttaaaa
     3601 atctgtgagt atcgcaacga aagcacaact tgaggcatgc gcctttaaac caaccgtaac
     3661 caaattcgga ggcaaaaaaa gatttctcgc tgttttttca gttttaaaat cacttttcac
     3721 gtttcttttc gcagtaattc gtttgcaaaa catctgaaaa aattttattt tataagaaat
     3781 tatataaaaa tcgctataaa ttgagttttt gcccccaaat tcagcacgga gcacttctca
     3841 attaaatata attttatttt ttaacattga aagctttcat aaaattgagc tttgtttgta
     3901 aataatcagt gaaaaaacac agaaatcttc aaaaaataaa gaaaaagttt ttaaaaatct
     3961 gtgagtcccg caacgaaaat aaaactttag gcatgcgcct ttaaaccaac cgtaaccaaa
     4021 ttcggcggca aaaatggatt tctcgccgtt ttttcagttt tacaatcact ttttaggttt
     4081 ctttttgcag tatttcgttt cccaaacaat taaaaatcaa attttctttt ccagatggct
     4141 caaacaaagc cgattgccga gcaaatggga tcccccgggc tgcaggaatt cgatatcaag
     4201 cttatcgata ccgtcgacct cgagggggcg gtaccggtag aaaaaatgag taaaggagaa
     4261 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     4321 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     4381 tttatttgca ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta
     4441 actaaccctg attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt
     4501 caatgcttct cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
     4561 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     4621 cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa
     4681 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     4741 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     4801 atcatggcag acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta
     4861 actaactaat ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga
     4921 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     4981 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     5041 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     5101 gaactataca aatgcccggg ggatcggtgg agctccaccg gtggcggccg ctctagaact
     5161 agatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     5221 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     5281 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     5341 ataatgtgtg gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa
     5401 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     5461 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     5521 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     5581 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     5641 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     5701 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     5761 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     5821 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     5881 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     5941 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
     6001 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     6061 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     6121 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat
     6181 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     6241 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     6301 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     6361 atgaacggct cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac
     6421 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     6481 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     6541 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     6601 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     6661 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     6721 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     6781 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     6841 tttctcgttc agctttcttg tacaaagtgg ttgatctagt ggatcctaat tttgccgtat
     6901 tttccatatt ttgttttgta tatttatcca ctcaccccct ctctttgtcc tgtgaatgaa
     6961 cttgtgccaa naaaagccan aaaataattt acttttttta aagacatatt aaaacttgaa
     7021 aacttggaac aatacggtgc gaaagcgaaa cagagacaga aaaatgaaca aagagatccg
     7081 tgaaaagtga acgatgattt aggcttcttg ttgggcggcg tggatgagag tgatgttgtc
     7141 tcctttgaga agaattctgc cgatcttgtt gcgtcccttc gtcttcatgt tcacctcctc
     7201 ggcttcgtcg aacacgacat tcatgaactc gtcgaatccg atgatgtagc cctcgagacg
     7261 gtgggtgaca tcctcgtaca accagatttg gacccgagtg cggttttgga gatatctgaa
     7321 atttgggaaa ttgttttcta ctgattagtg aaaaaatatt acctgaagat gaggttcaca
     7381 ggctgaacca tcactttgtt gagctttctc gtcgacattt ttgcgggatg atctgtaaaa
     7441 atcaatatat ttntttgaaa gacacaaatt tttaagataa atatcaagaa aactcgattt
     7501 tagcagctat ttaagctcaa aaactacaat ttatggcttt aaaacataat ttttcacatt
     7561 ttacataaat ttcaacgaaa aaaaatgcca aaaaacttca aatttgagca gaaatcggct
     7621 attttctcga caaaaataat ttcgaaaatt cgttcaaaaa agatgagtac gcaagcaccg
     7681 tgacgcacag ttgcggactt tgtaaaccgt acccctgcat aaaaaggcgg aattttgaat
     7741 ttaataaaat gcagtttaga acaaagaaaa gcacggagag aattgtgata actaattatt
     7801 tgtatttaat tttaaatact acataaaact gaagattgga acgagttaaa gctggtattt
     7861 gcggtttata gccccaaaag ttaaggcttg ttaggcaatt atcgataaaa acaacgagaa
     7921 attcgtgaaa tcggcaaaaa tttatagaaa acgtttgaat taaattaaat ttacgctttt
     7981 gccgccaaca cctatcgaga ccctagttat aggggcggag cgtcantggg agcgcgcttg
     8041 caactcgatt tcaaaaagtt tcgctgtttg tacaacattc tcagttgttc ttaatcagtt
     8101 aaataagttt caaaattttn ncaaaatcat aaaacacagt taatgctaaa ttaaagtcta
     8161 aaaanatcag gtgataaggg gtttggacga ccaaaaagcg accttcgctc gaaaattgat
     8221 aaactaacta cactttttcc ggaaaaatga tggaaaaatc ctgattttct tcgttgaatc
     8281 aatttttgtt tcatttttaa cactttcaca tgannaatat ttcagttaaa atgctcaagc
     8341 taattgggtt tcttataatt ttgggtctcg caacggccga caagtgctca gattgtttgg
     8401 ctcaggggaa acagtgagtt ggaagcttac aattctgaga attcgtattg cgcagcttat
     8461 ttgacgcgca aaatatgaaa tatcacgtag cgaaaactac agtaattctt taaataagac
     8521 tactgtagtg cctatgtcga tttactgcct caactttctt aatcaagctc gtaaatcgac
     8581 accagctcta cagtagtcat agttttcgct acgagatatt ttgcgcgtca aatatggtgt
     8641 gcaatacgca ttttcagaat tttatgttct cgtaatatac cttttttcag ctggtgtgtt
     8701 actgtaaatg catgtggatt tacgccatgt gtcactacaa ttacagtgag ttttttntga
     8761 aganaaaaaa aattacccat ttgatcgcca attccatttt tcaaaaatca ataaaaaaaa
     8821 aaaatttcgg cgatcagaaa cgttacagaa agccctaaac tgcccggaac taccagataa
     8881 agcccacgct tacgatgatt catttatgag gaataaggta ttgatcacac attctgctgc
     8941 ctacaatatg gacccacaaa agtgttttac gtaggttcac cttctagata aaccttaaaa
     9001 atcgatactt tcagtagtaa aatgccttcg atgaaagttt ccaaaacagt taatgtcaat
     9061 tgtgatagat atgggnccaa gtgnagttgt tttattagcc cacggatttc tggcttccct
     9121 cataaattga aaaggaagag attgccgaac tagaccattt tggctcggcg cgttgcgtgt
     9181 cacgtcgcgg ctcgattgta aaactaaatg tattggtccg tgtggagtac acgactttcc
     9241 ctttccggca ggcgattgtc aatggagcgc gaaaaattca atgaggaagg ccagaacccc
     9301 gtgtctgtag ttttctcaaa aagtggcaaa aactcaataa ttgccaattt ttgactgcca
     9361 ataaaaattt cactaaattt attaaattta ttttaagttt tattgaaatt ntttttaaaa
     9421 tttccccact ggcgctaaaa aatccaattt tcagaagcaa tgtgcttcag cttcacggcc
     9481 atagatactg tccaaaaagt gattgcaatg acgtttagag ggacagaagg agcagctcaa
     9541 ttgtctgaag aaattttgga tttcttccag taagttcggg aaatggcaat ttaaagttta
     9601 attggatttt tgtatttttt cttctcacga ctacagtttt gcacattttt atgttataaa
     9661 aaaccaaaaa aaaatttttt tgccnttttt tctcagctta ccacaaaaca atttcgacca
     9721 aaaataaatt gaaaaaatta aaaaattttt tgcacggttt tcggactcaa aaaaagctga
     9781 atgttcctgg aactatttgt aaaattcaaa attcaaaatt ttcacgaatt ctctaaaagg
     9841 ttccagcaaa ttccacaact ttcttgaatt tttcaaaaaa gttcttgagc attcaacaat
     9901 tatttttgga attttccaaa ggttacagga cgttccaatt tttcaaaaaa atttctcgca
     9961 aaaaatnngt tttctcagaa aatttgaatt tccctccaca taaaagattg ttctcaaaaa
    10021 atttgcaaac acacattttc agcnggaaaa aaggcgttct tcgactctgg aagtgtattc
    10081 gaatatggta cccagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc
    10141 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatagg
    10201 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac tcacattaat
    10261 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10321 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctcgg
    10561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10621 actataaaga taccaggcgt tcccccctgg aagctccctc gtgcgctctc ctgttccgac
    10681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10741 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10981 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11341 gatctgtcta tttcgttcat ccatagttgc ctgactgccc gtcgtgtaga taactacgat
    11401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11701 atcccccatg ttgtgaaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11761 taagttggcc gcagtgttat cactcatgct tatggcagca ctgcataatt ctcttactgt
    11821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    12001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    12061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
