LOCUS       sequence-127643-        6451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a 
            level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pPML1 (GB0045)
FEATURES             Location/Qualifiers
     source          1..6451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3501..3519)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3502..3519)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3537..3553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3537..3553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3550..3572)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3561..3577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3585..3591,3592..3609,3610..3615))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3630..3651
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3768..3785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3939..4527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4019..4038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4698..5489,5490..5558))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5321..5340
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5559..5663)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5741..6196)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5878..5899)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6090..6109
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6322..6344
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6336..6353
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6337..6353
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6360..6379
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6360..6378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6409..6418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6413..6422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcattag cttaaccggt ggattcaggg agtttcttta accagagaaa ggtagaatat
       61 atcactcttc agaagaaaac aaatggagga tagttaacga aataaatgga gaatttggtg
      121 gaggaggaac ttaggagaaa gggaagaaga gaaagaggtg aggaagagga tgtggcagaa
      181 ggaaaaggag ttattgcgaa atgcatagag agggtgcatt gcatttatga ttatcgaaag
      241 gtccattaat gcttttagtg agagaaaatt catactacta cttatatatt tttctagaga
      301 gagagtttta cagttcatca atttaaaaaa caaaatacaa aaaaaaaatc actgtttccc
      361 tttgacctca cggattctcg tagggtaaga aaaaacaacc gaataagaat ttgaacaaat
      421 aaaaatgaag aaacattacc atttcattgt tattatatat gcttgtggtt atagttcatt
      481 ttagattttt ttagtatatg tatgatgtat catatgatca gaaagagtat ttttatttct
      541 gtgtgtggag catattgaac attgattgca tgtgtgtcca atatatgtgg acatataaat
      601 gctttgtatg gtttttaaat actcacaatt agtatacaca ttatatcagt caataatgtc
      661 catcatgagg ttcctaattc attgaatata taatcctcca taaattagta tagtgtttga
      721 gcttaattat tattcgaata cctgcaggtt ttattagtac tagcttcttt tgatggttga
      781 ttggtgatga gattaagtag aatagcaaga acattacagg actacattct taggtcctca
      841 ttatacattt atactgttat aaaagtaact aaacaattta atctcaaaat aagctagtta
      901 gattctataa aattaaacat ataaaataaa aagattctat aaaattcaaa ggtaggtgct
      961 aatttaagag attaatttaa agtttcaaaa tccatgacat tgacacgacg atcgccataa
     1021 aaataactaa gcaatctaca aatttcctag attcttaatc ataattttta attgcacatc
     1081 gctattaatg gtccacccct cccttattat acaccctcac ttatatccta gatttctaaa
     1141 ttcgtattta atcagtacat aaaatattaa atactaaaag ctaatccaat agtttttaac
     1201 caaattttta tcttttggct gatgtcaaaa tgaatttatc ttgttggcta gagtagtaat
     1261 tatagtatac actgatgtta attaaatttt tgaacctata taattatgag tttttaaaat
     1321 acaaaataca aaatataaga tcattttttg tgattggacc cactttcaag gagcaaaaga
     1381 aagggatgag atgcatgtaa actacgtaag ttccaataat atctaaaaat gaatagaaaa
     1441 aaggtcaaag ttatatttaa tggtagtttg gattaattat cttaattaaa aattggccag
     1501 tcgattgact aatgtagttg gggagcaata tccattgtgg accatggtcc aagaaacagc
     1561 tcatacatta agatttacat cacctacatc atatacgtac actgactgat tcagacattc
     1621 atgtaacata catgaataca catacatgca catacgattt attattggat ttacctgacg
     1681 tgttatttgt gtggtattgg attaacctat gctttgtttg cacatactat atatgttttc
     1741 aacaagaaaa tgacaagatt cacttttaca aatgttcata ttcatatata gtacataata
     1801 cacaagaaaa aaaacggcag cctactaatc aatgtaaaca ctaaacatag tagatacttt
     1861 acaaaaatca ttgcgcaaaa gcataagttt ttcgtaaatt acaatttgat ataaaaccaa
     1921 cactttttta ttattatttt ctaatatgaa atagtcaaat atagctccca aaatattaaa
     1981 tatgttgagg gtcctatgtt tctttctgac gccacgaccc tgcatttgca tatgcataaa
     2041 tatgcaatgg ctattaagta tttgtgtagc aacccaatat ctcttggttg gaaaaagttg
     2101 gttgacactc gtcttatttg gaaaaggttg aaaatttgtt cacaagttcc tgattagaca
     2161 gtagtacccc atttggtcga cactcatcat ttttggaaaa tggtgaaatt tggttctcgg
     2221 attcgagttt tgacagttga aaatggccta agtatatgat tttatgttcg aaaagaatta
     2281 tggatcaaca atataaagtt acttgacttg taatagtaaa ataaatggtc cgcgattaat
     2341 tgtggagagg tacattacac atatgcaaat ttatgtccac ttttaatgtg atatatatta
     2401 tgcagtcatt catccaatat ctattaagtt taataatact agctgtgttt ccttgcattt
     2461 gtcgtcttcc tcgtgaattt ttagatatat ttgctctctt tctaatcggc ttttaagttt
     2521 gattatgatt ttaatatgac ttagtagtat ggatgacgaa ttacttaact tattccgtta
     2581 atgatgctga tcatatgata ttaaaatcaa gtttattatg taccactgtg tgtgtgtgat
     2641 tccttgcttg aagttgttac ttagaaaaat tgattgtagc atactataaa gttttagaac
     2701 tgatacactc aatgtagctt atcattgtta attaaactca cgtagaggaa acaattacta
     2761 attaatttga tataatacgt ataaattctg tatcgaaata tatataagtc tagttgattt
     2821 atatacaaat ttctcgatgc ttagttgttc ctatctatat cttatacata gataattcat
     2881 cacttgaaat tttgtaatac gagttaagcg tattgtttga aggggtgtgt tagcgataat
     2941 ccaaatacgc atggtgcgaa tcaaataacc gacatgtagt ccacttagtc tagaaataaa
     3001 cgacaggatg tgtaaatggg gcattggtac aagaggtgat tttttataga tatgaggagg
     3061 gtaattcagt aaattaaagg aactgaaacg ccttggagtt gtggggacgt ttcttctaat
     3121 aataatagct agtgggaacg agtgacttct gcctaccaac taactcattt attgccttca
     3181 aatttcgttt ttgttttgtt taaaattaaa aaccaaaaac caatttccaa attcagaaat
     3241 tcgattttat tttatttatt tgctttggtc cagtgaatga ttattttcag taatttgaaa
     3301 caaagtttca gacaatgaat catgtttcag tttctgaatt ttagcacatt ttagaaacta
     3361 tgcatcgttt tgttcttgtt ttttctttga taagcttact cccgagacca ctcatcgccc
     3421 aatcactagt gaattcgcgg ccgcctgcag gtcgaccata tgggagagct cccaacgcgt
     3481 tggatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
     3541 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3601 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3661 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3721 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3781 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3841 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3901 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3961 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4021 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4081 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4141 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4201 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4261 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4321 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4381 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4441 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4501 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4561 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4621 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4681 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4741 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4801 ggcttaccat ctggccccag tgctgcaatg ataccgcgcg acccacgctc accggctcca
     4861 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4921 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4981 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5041 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5101 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5161 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5221 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5281 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5341 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5401 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5461 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5521 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5581 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5641 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga tgcggtgtga
     5701 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
     5761 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     5821 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     5881 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     5941 gtctatcagg gcgctggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     6001 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     6061 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     6121 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     6181 ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     6241 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     6301 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
     6361 aatacgactc actatagggc gaattgggcc cgacgtcgca tgctcccggc cgccatggcg
     6421 gccgcgggaa ttcgattggg cgatgagtgg t
//
