LOCUS       sequence-127644-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with
            an N-terminal EGFP tag and C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet 
            P, Ramaekers FC, Bornens M, Grand-Perret T
  TITLE     Autophosphorylation of polo-like kinase 4 and its role in centriole 
            duplication.
  JOURNAL   Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 
            10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
  PUBMED    20032307
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-06-0505"; journalName: "Mol Biol Cell"; date: 
            "2010-02-15- 15"; volume: "21"; issue: "4"; pages: "547-61"
COMMENT     Sequence Label: pEGFP-C3-PLK4 K41M-3xFLAG
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     154..175
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
       61 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
      121 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      181 cgccgggatc actctcggca tggacgagct gtacaagtac tcagatctcg agctcaagct
      241 tggtaccatg gcgacctgca tcggggagaa gatcgaggat tttaaagttg gaaatctgct
      301 tggtaaagga tcatttgctg gtgtctacag agctgagtcc attcacactg gtttggaagt
      361 tgcaatcatg atgatagata agaaagccat gtacaaagca ggaatggtac agagagtcca
      421 aaatgaggtg aaaatacatt gccaattgaa acatccttct atcttggagc tttataacta
      481 ttttgaagat agcaattatg tgtatctggt attagaaatg tgccataatg gagaaatgaa
      541 caggtatcta aagaatagag tgaaaccctt ctcagaaaat gaagctcgac acttcatgca
      601 ccagatcatc acagggatgt tgtatcttca ttctcatggt atactacacc gggacctcac
      661 actttctaac ctcctactga ctcgtaatat gaacatcaag attgctgatt ttgggctggc
      721 aactcaactg aaaatgccac atgaaaagca ctatacatta tgtggaactc ctaactacat
      781 ttcaccagaa attgccactc gaagtgcaca tggccttgaa tctgatgttt ggtccc
//
