LOCUS       sequence-127652-        3357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2). ApR, expression vector with 
            rrnBT1T2-Pm-MCSII, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 51
FEATURES             Location/Qualifiers
     source          1..3357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      136..222
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        317..397
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     rep_origin      901..1359
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(988..1007)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1201..1222
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      complement(1469..2057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1549..1568)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2228..3019,3020..3088))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2851..2870
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3089..3193)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3261..3279
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3327..3346
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3327..3345
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgcgc gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
       61 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
      121 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      181 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
      241 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
      301 gaatttgata gagataagtc cagccttgca agaagcggat acaggagtgc aaaaaatggc
      361 tatctctaga aaggcctacc ccttaggctt tatgcaacag aaacaataat aatggagtca
      421 tgaccatgac aatgcacctg gggctcgact atatagatag tctcgttgaa gaagatgaga
      481 acgagggcat ctaccgctgc aagcgcgaga tgttcaccga ccctcggctg ttcgatttag
      541 agatgaaaca catctttgag ggcaactgga tttatctcgc ccacgagagc cagattcccg
      601 agaagaacga ctattacacc acgcagatgg gccggcagcc gatattcatc acacgcaaca
      661 aagatggtga gctgaatgcc ttcgtcaatg cctgaattcc caattcggtt cacccgggca
      721 tatggcaaga actgcagata tccatggtac cgagctcgga tccgtcgaca agcttgcggc
      781 cgcccagctt gctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag
      841 ctcccgctct gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt
      901 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      961 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     1021 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1081 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     1141 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1201 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     1261 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     1321 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc taaccgatcc ccctggagct
     1381 tggaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1441 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1501 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1561 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1621 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1681 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1741 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1801 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1861 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1921 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1981 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2041 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2101 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2161 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2221 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2281 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2341 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2401 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2461 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2521 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2581 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2641 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2701 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2761 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2821 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     2881 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2941 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3001 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3061 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3121 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3181 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     3241 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattgg
     3301 tcgcatcgat gctggcttat cgaaattaat acgactcact atagggagac cggaatt
//
