LOCUS       sequence-127660-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with 
            an N-terminal EGFP tag and C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet 
            P, Ramaekers FC, Bornens M, Grand-Perret T
  TITLE     Autophosphorylation of polo-like kinase 4 and its role in centriole 
            duplication.
  JOURNAL   Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 
            10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
  PUBMED    20032307
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-06-0505"; journalName: "Mol Biol Cell"; date: 
            "2010-02-15- 15"; volume: "21"; issue: "4"; pages: "547-61"
COMMENT     Sequence Label: pEGFP-C3-PLK4-S305E-3xFLAG
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..197)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gttaacacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat
      121 ccggtggatc ctttgtcatc gtcatccttg taatcgatat catgatcttt ataatcaccg
      181 tcatggtctt tgtagtccat cgtcgactgc agaattcgaa gcttatgaaa attaggagtc
      241 ggattagaaa acatcaaaag gatggaagac agacactgta atttctgttt gatgtagtct
      301 ggtaattttt cattttctcc atacctagtt gtttgaccat ttggtgaggt ataactgata
      361 gaagacactc ctgcctgcac aaccaactgg gacccatcat taaactgaac ccacacagct
      421 ccactagtta actgtgtagc ccaaccaaca tttttcacaa aaacagattt caaaagttgt
      481 gctgatttag gaagacaatc ttttagacta ttagaagaga tgtctgttcc agaagctgta
      541 gttgtaagac caagtccttc atttgtaacc atagagggat taaggattgg tgcctgtgtt
      601 ggagaagcag cactatgcat gaccattctg ttgaaagatg ctctatctct cgttgggtaa
      661 tttgagtcca cagaaggagg aggtgataag gccttaggtg aactagtact accaggtttt
      721 cttcctatga ttattgggaa aaagggagca ctcctagttt tcctttcctc ttctgaaatt
      781 atggattcca gtgctaaaca aatacgatga ccctcattag catggtccat atacattttt
      841 atctcctctt tcaagctatt aacttcactt
//
