LOCUS       sequence-127661-         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII-sspH2 signal peptide, M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 56
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             513..535
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gaattggcga taagcttgtc gacggatccg agctcggtac catggatatc tgcagcccgg
       61 atgccccttc cgcgtgtcct gtgccctcct caccctgtgc tgtgtccagg gtgaggcgat
      121 gtgtttcact gtacccctgg caatttacgg tatagttccc ggcatcatca agggtgactg
      181 acaatatctc ctgactgtct tcatccagaa tacagaagta attttccccg tgctggccgg
      241 aatgaatgct ttcctcccat ccggcatacg cgagcgtcct gagcagttca aatctgttga
      301 tcacatcctc ccgcgtcgtt ccggccggcg ggtgacaaat cgtccagatg cactccagcg
      361 cttcagtctg gtgcgttgag caaaaaaatt ccttcatttt ttcccaggaa ctcatttcag
      421 ggggggtatc agaccaggca atacgataaa tgcggcgatt actgatggtg gcgggaagac
      481 atccgcttcc aatatgaaag ggcatatgta tatctccttc ttaaagttaa acaaaattat
      541 ttcccgggtg aaccgaattg ggaattcagg cattgacgaa ggcattcagc tcaccatctt
      601 tgttgcgtg
//
