LOCUS       sequence-127670-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF
            attenuator-T7 SD sequence-MCSII- sspH2 signal peptide, M13 replicat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 62
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             536..558
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgtaatacga ctcactatag ggcgaattgg cgataagctt gtcgacggat ccgagctcgg
       61 taccatggat atctgcagcc cggatgcccc ttccgcgtgt cctgtgccct cctcaccctg
      121 tgctgtgtcc agggtgaggc gatgtgtttc actgtacccc tggcaattta cggtatagtt
      181 cccggcatca tcaagggtga ctgacaatat ctcctgactg tcttcatcca gaatacagaa
      241 gtaattttcc ccgtgctggc cggaatgaat gctttcctcc catccggcat acgcgagcgt
      301 cctgagcagt tcaaatctgt tgatcacatc ctcccgcgtc gttccggccg gcgggtgaca
      361 aatcgtccag atgcactcca gcgcttcagt ctggtgcgtt gagcaaaaaa attccttcat
      421 tttttcccag gaactcattt cagggggggt atcagaccag gcaatacgat aaatgcggcg
      481 attactgatg gtggcgggaa gacatccgct tccaatatga aagggcatat gtatatctcc
      541 ttcttaaagt taaacaaaat tatttcccgg gtgaaccgaa ttgggaattc gcgcaaaaaa
      601 aaagcgcccg gcggtgctta cgcaccgctg gacgccttta tccctggaca tacgcgccgc
      661 cattg
//
