LOCUS       sequence-127675-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 52
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(204..308)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     376..394
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     442..461
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        442..460
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      608..694
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
       61 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
      121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
      181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
      241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      301 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
      361 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attggtcgca
      421 tcgatgctgg cttatcgaaa ttaatacgac tcactatagg gagaccggaa ttgcggccgc
      481 gcgaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
      541 gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc
      601 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      661 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
      721 gaagcaacgg cccggagggt ggcgggcagg a
//
