LOCUS       sequence-127680-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 52
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(54..512)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(191..212)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     406..425
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     RBS             699..721
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc caagctccag ggggatcggt tagaaattgt
       61 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
      121 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagcccg agatagggtt
      181 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
      241 agggcgaaaa accgtctatc agggcgatgg ccgcccacta cgtgaaccat cacccaaatc
      301 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
      361 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
      421 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
      481 cgccgcgctt aatgcgccgc tacagggcgc gtactatggt tgctttgacg agcacgtata
      541 acgtgctttc ctcgttagaa tcagagcggg agctaaacag gaggccgatt aaagggattt
      601 tagacaggaa cggtacgcca gcaagctggg cggccgcaag cttgtcgacg gatccgagct
      661 cggtaccatg gatatctgca gttcttgcca tatgtatatc tccttcttaa agttaaacaa
      721 aattatttcc cgggtgaacc gaattgggaa ttcaggcatt gacgaaggca ttcagctcac
      781 catctttgtt gcgtgtgatg aatatcggct gccggcccat ctgcgtggtg taatagtcgt
      841 tcttctcggg aatctggctc tcgtgggcga gataaatcca gttgccctca aagatgtgtt
      901 tcatctctaa atcgaacagc cgagggtcgg tgaacatctc gcgcttgc
//
