LOCUS       sequence-127686-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of rat GAT with N terminal YFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Scholze P, Freissmuth M, Sitte HH
  TITLE     Mutations within an intramembrane leucine heptad repeat disrupt 
            oligomer formation of the rat GABA transporter 1.
  JOURNAL   J Biol Chem. 2002 Nov 15;277(46):43682-90. Epub 2002 Sep 9.
  PUBMED    12223478
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-11-15"; volume: "277(46)"; pages: "43682-90. 
            Epub 2002 Sep 9"
COMMENT     Sequence Label: pEYFP-C1-rGAT
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(98..117)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             257..508
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     445..466
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
       61 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      121 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      181 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      241 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
      301 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      361 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
      421 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      481 cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcgaa
      541 ttctgcagtc gagtggactg gagctgacat cgcacgcctg cccgccagga tccctgctgc
      601 caagttcgtg ctccgagaca tggcgactga caacagcaag gtggctgatg ggcagatctc
      661 taccgaggtc agcgaggctc ctgtggccag tgacaagccc aaaacccttg tagtcaaggt
      721 gcagaagaag gctggagacc tccctgaccg ggacacatgg aagggacgct tcgacttcct
      781 catgtcctgt gtgggctacg ccatcggcc
//
