LOCUS       sequence-127691-        7522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with SLC6A4 (Ple198) promoter driving expression of 
            iCre..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1997
FEATURES             Location/Qualifiers
     source          1..7522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     CDS             590..601
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     intron          3327..3459
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3442..3461
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    4540..4661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4551..4570)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4605..4624
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4830..4959
                     /label=AAV2 ITR
     primer_bind     complement(4978..4995)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4978..4994)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4987..5009)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5136..5591
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5223..5242)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5433..5454
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5617..5721
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5722..5790,5791..6582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5940..5959)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6753..7341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7242..7261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attccctagg ccggccacat gtgggcctac
      481 gcacatcttt gctgagggag tgcttctcca tgaagaaggc cagattccat gtttactgct
      541 tcgggcggca ccagcgttcc ataattccca tcactcttgg gagatgccct gggaacttca
      601 gcctccctct gaatagagga tacgaactgc cagttggctc tatgacctgt aactaatcct
      661 atctcaacgt taaacccaca gttcatttgt ttttgtttgt tttgagaaag aaagttgagt
      721 ttcctgagaa atgaactcac tttgatcttg acacatgaag tcaaagttca acatttctgt
      781 agtgtctttc tgcttagaag gcactgatag gcatcaagta gatggagcgt cacaggcatt
      841 tccaaagggc tgggtgttta tgaccaatcg ggataattac atgcttgttc aggtacgggt
      901 tgctcctggc tatagagaca aagtgtgtgg aaagaggaat ggagattcga tcagaagttt
      961 aaatagaaac agtccttttt tacttggagt cagggattgg gggaatggag tgcattagct
     1021 tcaggagagt taagagaaag tcaaccactc ccattggctg gtcaactgca cccctcatgt
     1081 gcagagacct agggggacga agcttctgtc tgccagctgt cattccacag cagaggtggg
     1141 gtccacggca ccaccctcag cctgtcacca gctcagcctg gaacaacagc acacatccaa
     1201 gatgcaaagg aaggcttcct tctggggctt tttctggaca tggagaaact caaagactgc
     1261 ctggagaagg tcacagctgt aatattgacc tcgtttgcaa tctttccacc cagcaggata
     1321 aaaatacctt ttaaaaaaag aaagcttaga agagggcatt ctcagaaacc aaccttctta
     1381 acaactcaca cttctctatt cctgggccag ctcgttttca gccattgtta tgtgcaagtc
     1441 ttgatagaaa aagccagcca gggctctgca gaatcagaaa aaataggaag agagtctggg
     1501 gctccacagg caggagcttg caggcagaaa gggccccggg cacatggcat cttctcctgg
     1561 cagacccgat tagtcaccga gaggaggcct ggtaaatggt tacctatggt gggtgagagg
     1621 atgtgtggtt attttaaaag cctatcaagc ctagttgagg gccgaaggaa gggggaagtg
     1681 gggggaggcc aaggccaggc agtagcataa atggtgagca gggtgaggtt atggagaccg
     1741 ctcttgtcag aattcatatc cttctgagtg gtgtttgcat tcttgagcct ggggtgggtg
     1801 gcggagggcc caacaagtgc ggtacagccc aagacgtcta cctcccctta cacacacaca
     1861 gcccctagct acatgaagct gatttatctc ttgttgctgt agatattttt ggcaacaatc
     1921 gatcccttta aaatttttat tttccacttt acagaaaaaa tgcagcaaac gccaaaccct
     1981 agtgactgac attgcctggt gttcatgtct aatccttgct catgtttata gccatagtca
     2041 ttcttgtgaa gctactattt tgtgtcctga tttttcactt actatgtttt tgtcttcaca
     2101 tgtgtaggta gctaagttgc agtcaccaaa aaccccaaat aggtaggaga catccatgcg
     2161 ccatttctat tagccagttc ctagccatcc tcctaactgt tggatctgga gggttagcct
     2221 cagatcattg cagttataaa cagtccatcc ctggctatct tttctagaag ggtccagtgt
     2281 catctcagct aggcagaaac gtatttcaac tcccaaactg tttcaagagt ttgaaaagct
     2341 caggcccaga agaaacagag tgagcagttg aacagtctta caactgctgc ttacaacttt
     2401 ataacaggcc ataggtttaa agtccaaaag ggctcaaatt tctaccccac gtttgacgac
     2461 tcagacctca agccagagct gagctgactt ccctgcgccc aggccacggc ggtgaaatga
     2521 aggcacagca gccccggcca ggccctccca gtggaggcac agggggacgg cgacaccaac
     2581 caccccagcc cagttcttca ttccttcatt gtggaagtct ttattgtgag actctgggga
     2641 cggagaggaa ttagacaagg gcctctaagc tgagctcaca tcccagccgg tcagtcagat
     2701 aaacgcatgg gtatcgagta ctgctaggtc ccaggaagaa agagagagca gctttcggga
     2761 tggggacgat ggggaggtgt ccgaggtcaa gagaaagcgg cacgagcaga cccctgtgtg
     2821 ccgtcctgtg ggcgcggggc ggcaggggag gcgcacacct gctcctttgt gcagcctccc
     2881 ccctcccgca aagttaaaga gcaggaaagt caggattcct cgctcggccc tgccctgccg
     2941 gctgctccgc gctccgctcc tccctgcgag cgtgtgtgtg tgtcgggggt ccctcccctc
     3001 ctggctctgg ggtcgggcgc gcaccccgcc ccgtagcgcg gcccctccct ggcgagcgca
     3061 accccatcca gcgggagcgc ggagccgcgg ccgcggggaa gcattaagtt tattcgcctc
     3121 aaagtgacgc aaaaattctt caagagctct ttggcggcgg ctatctagag atcagactat
     3181 gtgagggccc gcgggtacaa atacggccgc gccggcgccc ctccgcacag ccagcgccgc
     3241 cgggtgcctc gagggcgcga ggccagcccg cctgcccagc ccgggaccag ccggcgcgcc
     3301 gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag
     3361 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
     3421 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc caggcggccg
     3481 ccaccatgtc caacctgctg actgtgcacc aaaacctgcc tgccctccct gtggatgcca
     3541 cctctgatga agtcaggaag aacctgatgg acatgttcag ggacaggcag gccttctctg
     3601 aacacacctg gaagatgctc ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga
     3661 acaacaggaa atggttccct gctgaacctg aggatgtgag ggactacctc ctgtacctgc
     3721 aagccagagg cctggctgtg aagaccatcc aacagcacct gggccagctc aacatgctgc
     3781 acaggagatc tggcctgcct cgcccttctg actccaatgc tgtgtccctg gtgatgagga
     3841 gaatcagaaa ggagaatgtg gatgctgggg agagagccaa gcaggccctg gcctttgaac
     3901 gcactgactt tgaccaagtc agatccctga tggagaactc tgacagatgc caggacatca
     3961 ggaacctggc cttcctgggc attgcctaca acaccctgct gcgcattgcc gaaattgcca
     4021 gaatcagagt gaaggacatc tcccgcaccg atggtgggag aatgctgatc cacattggca
     4081 ggaccaagac cctggtgtcc acagctggtg tggagaaggc cctgtccctg ggggttacca
     4141 agctggtgga gagatggatc tctgtgtctg gtgtggctga tgaccccaac aactacctgt
     4201 tctgccgggt cagaaagaat ggtgtggctg ccccttctgc cacctcccaa ctgtccaccc
     4261 gggccctgga agggatcttt gaggccaccc accgcctgat ctatggtgcc aaggatgact
     4321 ctgggcagag atacctggcc tggtctggcc actctgccag agtgggtgct gccagggaca
     4381 tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg accaatgtga
     4441 acatagtgat gaactacatc agaaacctgg actctgagac tggggccatg gtgaggctgc
     4501 tcgaggatgg ggactgagcg gccgcgatcg cagacatgat aagatacatt gatgagtttg
     4561 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4621 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     4681 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
     4741 acaaatgtgg tagtcgaaat tcccgataag gatcttccta gagcatggct acgtagataa
     4801 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     4861 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4921 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc cttaattaac ctaattcact
     4981 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5041 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5101 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     5161 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5221 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5281 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5341 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5401 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5461 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5521 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5581 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5641 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5701 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5761 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5821 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5881 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5941 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6001 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6061 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6121 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6181 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6241 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6301 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6361 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6421 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6481 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6541 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6601 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6661 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6721 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6781 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6841 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6901 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6961 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7021 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7081 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7141 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7201 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7261 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7321 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7381 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7441 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7501 tcagtgagcg aggaagcgga ag
//
