LOCUS       sequence-127698-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of rat GAT with N terminal CFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Scholze P, Freissmuth M, Sitte HH
  TITLE     Mutations within an intramembrane leucine heptad repeat disrupt 
            oligomer formation of the rat GABA transporter 1.
  JOURNAL   J Biol Chem. 2002 Nov 15;277(46):43682-90. Epub 2002 Sep 9.
  PUBMED    12223478
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-11-15"; volume: "277(46)"; pages: "43682-90. 
            Epub 2002 Sep 9"
COMMENT     Sequence Label: pECFP-C1-rGAT
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    89..223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(113..132)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     167..186
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gatccggtgg atcccgggcc cgcggtacct aaccaagttc ctctttcaga ggttatttca
       61 ggccatggtg ctgcgccgaa ttcccgggga tccagacatg ataagataca ttgatgagtt
      121 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      181 tattgcttta tttgtaacca ttataagctg caataaacaa gttcacctag ggtcagactg
      241 tcccccatct cccctctctg catggtgatg gtgtctggac ccgccccttg aggtagttgg
      301 agacagagag tatcttcagt cttcaagggg tgtgggtaca gccagtccag ggtgccagtg
      361 taggggtgat gagggggctg tacccctaga tgtaggcctc cttgctggct gagctgccag
      421 cctgcggctg ctcaggacca ttctcagggc gcacgatatc ttcactgggc tgaatcatga
      481 cctggagacg ctgcttcaga gagcccttta gggtgaggaa catgtaagcc atgtacccgg
      541 ggatgagcac catggaggac agagccatga gccagcccac gccctggccc cacttgggga
      601 aaacgtagct tcccatggtg agtggtgtca tctgcacagc actgaagaga aacacgcccg
      661 ccacaatgat gggtgtgaaa aaggaccagc acagcttcca ccagatgcag ggcctggagc
      721 caaccatctc ctggatgttg tcatagaacc ggttgacacc ataaaaccag gaaatggaga
      781 cacactcaaa gaaaaccagg aacagcaggc tcatgccact ggcagagtaa taatcgaaca
      841 gtttgaagac att
//
