LOCUS       sequence-127702-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII-sspH2 signal peptide, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 53
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(133..237)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     305..323
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     371..390
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        371..389
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      537..623
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
       61 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
      121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      241 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
      301 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttggtcgcat cgatgctggc
      361 ttatcgaaat taatacgact cactataggg agaccggaat tgcggccgcg cgaatttgcc
      421 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
      481 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
      541 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      601 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
      661 ccggagggtg gcgg
//
