LOCUS       sequence-127704-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII-sspH2 signal peptide, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 53
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             601..623
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 taacgtgctt tcctcgttag aatcagagcg ggagctaaac aggaggccga ttaaagggat
       61 tttagacagg aacggtacgc cagcaagctg ggcggccgca agcttgtcga cggatccgag
      121 ctcggtacca tggatatctg cagcccggat gccccttccg cgtgtcctgt gccctcctca
      181 ccctgtgctg tgtccagggt gaggcgatgt gtttcactgt acccctggca atttacggta
      241 tagttcccgg catcatcaag ggtgactgac aatatctcct gactgtcttc atccagaata
      301 cagaagtaat tttccccgtg ctggccggaa tgaatgcttt cctcccatcc ggcatacgcg
      361 agcgtcctga gcagttcaaa tctgttgatc acatcctccc gcgtcgttcc ggccggcggg
      421 tgacaaatcg tccagatgca ctccagcgct tcagtctggt gcgttgagca aaaaaattcc
      481 ttcatttttt cccaggaact catttcaggg ggggtatcag accaggcaat acgataaatg
      541 cggcgattac tgatggtggc gggaagacat ccgcttccaa tatgaaaggg catatgtata
      601 tctccttctt aaagttaaac aaaattattt cccgggtgaa ccgaattggg aattcaggca
      661 ttgacgaagg cattcagctc accatctttg ttgcgtgtga tgaatatcgg ctgccggccc
      721 atctgcgtgg tgtaatagtc gttcttctcg ggaatctggc tctcgtgggc gagataaatc
      781 cagttgccct caaagatgtg tttcatctct aaatcgaaca gccgagggtc ggtgaacatc
      841 tcgcgcttgc agcggtagat gccctcgttc tcatcttctt caacgagact atctatatag
      901 tcgagccc
//
