LOCUS       sequence-127705-        3831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII-sspH2 signal peptide, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 53
FEATURES             Location/Qualifiers
     source          1..3831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      136..222
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        317..397
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             726..748
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     rep_origin      1375..1833
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1462..1481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1675..1696
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      complement(1943..2531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2023..2042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2702..3493,3494..3562))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3325..3344
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3563..3667)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3735..3753
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3801..3820
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3801..3819
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgcgc gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
       61 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
      121 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      181 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
      241 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
      301 gaatttgata gagataagtc cagccttgca agaagcggat acaggagtgc aaaaaatggc
      361 tatctctaga aaggcctacc ccttaggctt tatgcaacag aaacaataat aatggagtca
      421 tgaccatgac aatgcacctg gggctcgact atatagatag tctcgttgaa gaagatgaga
      481 acgagggcat ctaccgctgc aagcgcgaga tgttcaccga ccctcggctg ttcgatttag
      541 agatgaaaca catctttgag ggcaactgga tttatctcgc ccacgagagc cagattcccg
      601 agaagaacga ctattacacc acgcagatgg gccggcagcc gatattcatc acacgcaaca
      661 aagatggtga gctgaatgcc ttcgtcaatg cctgaattcc caattcggtt cacccgggaa
      721 ataattttgt ttaactttaa gaaggagata tacatatgcc ctttcatatt ggaagcggat
      781 gtcttcccgc caccatcagt aatcgccgca tttatcgtat tgcctggtct gatacccccc
      841 ctgaaatgag ttcctgggaa aaaatgaagg aatttttttg ctcaacgcac cagactgaag
      901 cgctggagtg catctggacg atttgtcacc cgccggccgg aacgacgcgg gaggatgtga
      961 tcaacagatt tgaactgctc aggacgctcg cgtatgccgg atgggaggaa agcattcatt
     1021 ccggccagca cggggaaaat tacttctgta ttctggatga agacagtcag gagatattgt
     1081 cagtcaccct tgatgatgcc gggaactata ccgtaaattg ccaggggtac agtgaaacac
     1141 atcgcctcac cctggacaca gcacagggtg aggagggcac aggacacgcg gaaggggcat
     1201 ccgggctgca gatatccatg gtaccgagct cggatccgtc gacaagcttg cggccgccca
     1261 gcttgctggc gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg
     1321 ctctgattct aacgaggaaa gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc
     1381 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     1441 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     1501 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     1561 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggcggccat
     1621 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     1681 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     1741 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     1801 cgaattttaa caaaatatta acgtttacaa tttctaaccg atccccctgg agcttggaat
     1861 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2341 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     2401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2701 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2761 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2821 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2881 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2941 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3001 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3061 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3121 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3181 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3241 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3301 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3361 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3421 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3481 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3541 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3601 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3661 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     3721 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttggtcgcat
     3781 cgatgctggc ttatcgaaat taatacgact cactataggg agaccggaat t
//
