LOCUS       sequence-127712-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked hphMX marker, integration into S. cerevisiae X-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2513
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    182..215
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(825..990)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     911..935
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
       61 gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
      121 gaaagactgg gcctttcgcc cgggctaatt atggggtgtc gcccttattc gactctatag
      181 tgaagttcct attctctaga aagtatagga acttctgaag tggggattta aatgcggccg
      241 cgctgagggt ttaatcgtct atgaggagac tgttagttgg atatcagtaa tgagacgaaa
      301 aagctcgaaa tgaatggata tattcttttt gctactggca actgttgaat atttaatgtt
      361 aaaacaaact aactgaggta tattcgtatc tgtatgtaca catatactat atacaggaaa
      421 agataagcaa gagagaggat atcaactacg agagcgatcg attatatatc aaaagctgtc
      481 cgctttgcca cccataatcg gcgcttagtt tcggagttca atcataattc taccacctta
      541 cactcaactt actctttaac tcctatagta taatatcgcc actgacccca tattaaaaaa
      601 tttttttgct cgatcttcta tcctctttag gttaattgtc gctgttattg tctagatttt
      661 ttctcggaga tggcgcatct atttgccgtc aaaagatcct ctcataccat attaagtaaa
      721 ttgcctccat ttctttttcc tcgggcagag aaactcgcag gcaacttgct ctcgaagtgg
      781 tcacgtatta agtcctcagc gagctcgcat ggaatgcgtg cgatgagcga cctcatgcta
      841 tacctgagaa agcaacctga cctacaggaa agagttactc aagaataaga attttcgttt
      901 taaaacctaa gagtcacttt aaaatttgta tacacttatt ttttttataa cttatttaat
      961 aataaaaatc ataaatcata agaaattcgc ttatttagaa g
//
