LOCUS       sequence-127715-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII-sspH2 signal peptide, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 59
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             581..603
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aatcagagcg ggagctaaac aggaggccga ttaaagggat tttagacagg aacggtacgc
       61 cagcaagctg ggcggccgca agcttgtcga cggatccgag ctcggtacca tggatatctg
      121 cagcccggat gccccttccg cgtgtcctgt gccctcctca ccctgtgctg tgtccagggt
      181 gaggcgatgt gtttcactgt acccctggca atttacggta tagttcccgg catcatcaag
      241 ggtgactgac aatatctcct gactgtcttc atccagaata cagaagtaat tttccccgtg
      301 ctggccggaa tgaatgcttt cctcccatcc ggcatacgcg agcgtcctga gcagttcaaa
      361 tctgttgatc acatcctccc gcgtcgttcc ggccggcggg tgacaaatcg tccagatgca
      421 ctccagcgct tcagtctggt gcgttgagca aaaaaattcc ttcatttttt cccaggaact
      481 catttcaggg ggggtatcag accaggcaat acgataaatg cggcgattac tgatggtggc
      541 gggaagacat ccgcttccaa tatgaaaggg catatgtata tctccttctt aaagttaaac
      601 aaaattattt cccgggtgaa ccgaattggg aattcgcgca aaaaaaaagc gcccggcggt
      661 gcttacgcac cgctggacgc ctttatccct ggacatacgc gccgccattg gcgcgctgcc
      721 caaaaccttt tattcactcg aattcaggca ttgacgaagg cattcagctc accatcttt
//
