LOCUS       sequence-127717-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 
            chromosomal location, USER site for cloning, amp resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2188
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      62..251
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    299..332
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcttattta gaagtgtcaa caacgtatct accaacggaa tgcgtgcgat cgcgtgcatt
       61 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
      121 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
      181 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
      241 gacgctcgaa gatcgcgtca gctgaagctt cgtacgctgc aggtcgacaa cccttaatat
      301 aacttcgtat aatgtatgct atacgaagtt attaggtcta gagatcccaa tacaacagat
      361 cacgtgatct ttttgtaaga tgaagttgaa gtagtgttgc accgtgccaa tgcaggtggc
      421 tattagatta aatatgtgat ttgttctatt aagtttcctg tataattaat ggggagcgct
      481 gattctcttt tggtacgctt cccatccagc atttctgtat ctttcacctt caaccttagg
      541 atctctaccc ttggcgaaaa gtcctctgcc aacaatgatg atatctgatc caccacttac
      601 aacttcgtcg acggttctgt actgctgacc caatgcatcg cctttgtcgt ctaaacctac
      661 acctggggtc atgattagcc aatcaaaccc ttcttctctt cctcccatat cgttctgagc
      721 aatgaaccca ataacgaaat ctttatcact ctttgcaata tcaacggtac ccttagtata
      781 ttcaccgtgt gctagagaac ccttggaaga taattcagca agcatcaata atccccttgg
      841 ttctttggtg acctcttgcg caccttgttt caagcca
//
