LOCUS       sequence-127720-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 
            chromosomal location, USER site for cloning, amp resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2188
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    125..158
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gtggggactc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
       61 gtcgaaagac tgggcctttc gcccgggcta attatggggt gtcgccctta ttcgactcta
      121 tagtgaagtt cctattctct agaaagtata ggaacttctg aagtggggat ttaaatgcgg
      181 ccgcgctgag ggtttaatcg tctatgagga gactgttagt tggatatcag taatgagacg
      241 aaaaagctcg aaatgaatgg atatattctt tttgctactg gcaactgttg aatatttaat
      301 gttaaaacaa actaactgag gtatattcgt atctgtatgt acacatatac tatatacagg
      361 aaaagataag caagagagag gatatcaact acgagagcga tcgattatat atcaaaagct
      421 gtccgctttg ccacccataa tcggcgctta gtttcggagt tcaatcataa ttctaccacc
      481 ttacactcaa cttactcttt aactcctata gtataatatc gccactgacc ccatattaaa
      541 aaattttttt gctcgatctt ctatcctctt taggttaatt gtcgctgtta ttgtctagat
      601 tttttctcgg agatggcgca tctatttgcc gtcaaaagat cctctcatac catattaagt
      661 aaattgcctc catttctttt tcctcgggca gagaaactcg caggcaactt gctctcgaag
      721 tggtcacgta ttaagtcctc agcgagctcg catggaatgc gtgcgatgag cgacctcatg
      781 ctatacctga gaaagcaacc tgac
//
