LOCUS       sequence-127724-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJH122 variant Gateway destination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  TITLE     IMCE attB donor plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJH123
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             353..658
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     537..556
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat gcgtatttgc
       61 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
      121 gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
      181 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
      241 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
      301 cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt
      361 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
      421 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
      481 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
      541 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
      601 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
      661 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactga atatgttg
//
