LOCUS       sequence-127725-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2189
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    176..209
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggact
       61 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      121 ctgggccttt cgcccgggct aattatgggg tgtcgccctt attcgactct atagtgaagt
      181 tcctattctc tagaaagtat aggaacttct gaagtgggga tttaaatgcg gccgcgctga
      241 gggtttaatc gagatctttg tgttcggtta cccggctcag atcctaactt cttcttttgg
      301 tatgtttatt cgtataagtt actgttgtcc acaggcaata ctctgcagaa aattaaaacg
      361 gcattaatgc taggacaacc agaattgtta ctactgtatg tgcgatagtt gataactgca
      421 acattatgcc cggtatattc tcaaaaaacc ctattactgc atacgaagaa atcgcaagag
      481 aaatctttcg gtttggaaaa gctcactgtg aggttccttg gagccaatag taatacagca
      541 caatccaagg aaaaatctgg cctatatgca aggaaggaga gatagtcaaa agcattcttt
      601 cccctagaag ttggtgcata tatggcatcg ttaaaacata ttacccccaa aatttcttct
      661 ctaaacgatg tgcttggcct ttgtt
//
