LOCUS       sequence-127726-         367 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJH122 variant Gateway destination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 367)
  TITLE     IMCE attB donor plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJH123
FEATURES             Location/Qualifiers
     source          1..367
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(70..76,77..94,95..100))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(125..249)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    290..337
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga tcctaactca
       61 aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg
      121 ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
      181 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag cttttttgta
      241 caaacttgtt gatatccatt gctgttgaca aagggaatca ggggatcttg aagttcctat
      301 tccgaagttc ctattctcta gaaagtatag gaacttcaga gcgcttttga agctaattcg
      361 agctcgg
//
