LOCUS       sequence-127727-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2189
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      74..263
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    311..344
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat ctaccaacgg aatgcgtgcg
       61 atcgcgtgca ttcatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
      121 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
      181 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
      241 agaaggtttt gggacgctcg aagatcgcgt cagctgaagc ttcgtacgct gcaggtcgac
      301 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc tagagatccg
      361 caggctaacc ggaacctgta ttatttagtt tatgctacgt taaataaaga cctttcgttc
      421 acataactga atgtgtaatg gccttgagat ttcaagcata ccaagttggt ggagacgggg
      481 tcgttacaaa agactcttta agccaagatt tccttgacag ccttggcgat agcatcgcca
      541 acctcagtgg tagagttaga accaccaagg tcaccggttc tgacacctgc atccaagaca
      601 tttctaacag cttcttcaag agccctacct tcttcaacca aatccaagga taacttcaac
      661 atcatagctg ca
//
