LOCUS       sequence-127728-        9757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pJH122 variant Gateway destination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9757)
  TITLE     IMCE attB donor plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJH123
FEATURES             Location/Qualifiers
     source          1..9757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(20..25,26..43,44..50)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    58..74
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     63..85
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     82..98
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     82..98
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    117..150
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     CDS             complement(678..1703)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(678..1703)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(2189..2236)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    2277..2401
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2442..2747)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2544..2563)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(3089..3748)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3663..3682
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3802..3808,3809..3826,3827..3832))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(3857..3981)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    4022..4069
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    4137..4170
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     primer_bind     complement(4205..4222)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4205..4221)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4214..4236)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             4726..5520
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4780..4799)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5390..5409
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     promoter        5566..6011
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(6012..6098,6099..7433)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     oriT            7787..7896
                     /note="incP origin of transfer"
     promoter        complement(8131..8221)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        complement(8415..8505)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      8865..9453
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9354..9373
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9607..9624
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    join(9741..9757,1..5)
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
       61 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgtgc
      121 cagggcgtgc ccttgggctc cccgggcgcg tgaaataagt ggctgcgcgc ataaagcggg
      181 tgatgggact aacgcctgac tagtaacata gatgacaccg cgcgcgataa tttatcctag
      241 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
      301 gtaaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
      361 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
      421 aaactttatt gccaaatgtt tgaacgatcg gggatccaga tcccggacaa tcgtgccctg
      481 gggaccaaca caatccagaa gggcctgaat cactgcgacc ggccctcccg cgacccagcc
      541 gagcgagctt agcgaactgt ggacgagaac tgtgccacca agcgtaaggc cgttctctcg
      601 catttgcctt gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa tcagctcttg
      661 ttcggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca
      721 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
      781 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
      841 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
      901 cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag
      961 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
     1021 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
     1081 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
     1141 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
     1201 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
     1261 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
     1321 ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc
     1381 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag
     1441 gtcaggctct cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat
     1501 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
     1561 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
     1621 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
     1681 gcggtgagtt caggcttttt catatcttgc cccccgggat ctgttgtaag gagtctagag
     1741 cttggaggtc tgattttctc agtctccaga gatgtgttta tataggtagt aacctttttg
     1801 atatcagcca caagtgtgtg ggaatcttat cttcggattt caattaggaa ttaaccttat
     1861 tgaattctct tgaaaggaag tccgcaaagt ggttgtcggt tcctttaata tgttcaacat
     1921 caaacgaata gtggctaagc catgcttgcc atctgatgtt tcttccaagt tttgaatctc
     1981 ctttgtaatt aaggttaaca aaactcttga aatgagtatt atctgtccta atcagaaaat
     2041 gaacaggagt tagataaata ctgaatttct ttatagtatt tattaccgcc aatgtctctt
     2101 tgtcattgct gtggtaattc ggcatgcctg caggtcgact ctagaggatc cccgggtacc
     2161 gagctcgaat tagcttcaaa agcgctctga agttcctata ctttctagag aataggaact
     2221 tcggaatagg aacttcaaga tcccctgatt ccctttgtca acagcaatgg atatcaacca
     2281 ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     2341 agattttgca taaaaaacag actacataat actgtaaaac acaacatatt cagtcactat
     2401 ggtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc
     2461 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
     2521 ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
     2581 atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc
     2641 agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac
     2701 agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct
     2761 gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc
     2821 ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt
     2881 gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg
     2941 tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat
     3001 acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc
     3061 tggcttttag taagccggat ccagatcttt acgccccgcc ctgccactca tcgcagtact
     3121 gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca tgatgaacct
     3181 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     3241 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     3301 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     3361 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     3421 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     3481 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     3541 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     3601 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     3661 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     3721 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg
     3781 gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg
     3841 tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     3901 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     3961 agcttttttg tacaaacttg ttgatatcca ttgctgttga caaagggaat caggggatct
     4021 tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca gagcgctttt
     4081 gaagctaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgccgcg
     4141 cccggggagc ccaagggcac gccctggcac ctctagagga tccccgggta ccgagctcga
     4201 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4261 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4321 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgataagct agcttcacgc
     4381 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
     4441 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
     4501 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
     4561 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
     4621 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
     4681 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4741 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4801 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4861 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg
     4921 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4981 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5041 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5101 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5161 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5221 gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc
     5281 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5341 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5401 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5461 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5521 gcgggactct ggggttcgct agaggatcga tcctttttaa cccatcacat atacctgccg
     5581 ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc
     5641 aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag
     5701 attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa
     5761 agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta
     5821 aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt
     5881 gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact
     5941 tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg
     6001 agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta
     6061 ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat
     6121 ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac
     6181 agcaaaaaaa tgaaaaatat caagtttctg aatttgattc gtccacaatt aaaaatatct
     6241 cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct gacggcactg
     6301 tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct aaaaatgcgg
     6361 atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt gacagctgga
     6421 aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat tctatcctaa
     6481 aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga aaaatccgtt
     6541 tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca actgcacaag
     6601 ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat tataaatcaa
     6661 tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat gaaggcaact
     6721 acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat aaaggccaca
     6781 aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc gaagaatctt
     6841 tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac
     6901 ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc ggtatgattg
     6961 agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca tctaacacag
     7021 taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca
     7081 ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat atttacatgc
     7141 ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa actggccttg
     7201 tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac ttcgctgtac
     7261 ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga ggattctacg
     7321 cagacaaaca atcaacgttt gcgccgagct tcctgctgaa catcaaaggc aagaaaacat
     7381 ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa taaaaacgca
     7441 aaagaaaatg ccgatgggta ccgagcgaaa tgaccgacca agcgacgccc aacctgccat
     7501 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7561 gggacgccct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
     7621 acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct
     7681 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg
     7741 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
     7801 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
     7861 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
     7921 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
     7981 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc
     8041 cctgctgttt tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac
     8101 tgaggggaca ggcgagagac gatgccaaag agctcctgaa aatctcgata actcaaaaaa
     8161 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     8221 aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg
     8281 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
     8341 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
     8401 cttctgtttc tatcagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     8461 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc
     8521 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg
     8581 aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac
     8641 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
     8701 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaaaagg
     8761 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8821 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8881 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8941 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9001 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9061 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9121 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9181 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9241 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9301 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9361 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9421 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9481 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9541 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9601 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9661 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     9721 ggcagtgagc gcaacgcaat taatgtgagt tagctca
//
